Mutagenicity of kaempferol in V79 cells

The role of cytochromes P450

I. Duarte Silva, A. Rodrigues, J. Gaspar, R. Maia, A. Laires, José Rueff

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The flavonol kaempferol is widely found in the diet and is directly mutagenic in some short-term tests, such as the induction of chromosomal aberrations in eukaryotic cells. The presence of exogenous metabolizing systems enhances its mutagenicity. We have evaluated the role of cytochromes P450 in the induction of chromosomal aberrations by kaempferol in V79 cells. The results obtained suggest that there is a time-dependent biotransformation of kaempferol to quercetin, by cytochromes P450, as assessed by high pressure liquid chromatography. Quercetin seems to contribute to the mutagenicity of kaempferol in the presence of microsomal metabolizing systems. On the other hand, the direct induction of chromosomal aberrations by kaempferol does not seem to depend on the production of reactive oxygen species.

Original languageEnglish
Pages (from-to)229-241
Number of pages13
JournalTeratogenesis Carcinogenesis And Mutagenesis
Volume16
Issue number4
DOIs
Publication statusPublished - 1996

Fingerprint

Cytochrome P-450 Enzyme System
Aberrations
Chromosome Aberrations
Quercetin
High pressure liquid chromatography
Eukaryotic Cells
Nutrition
Biotransformation
Reactive Oxygen Species
High Pressure Liquid Chromatography
kaempferol
Diet

Keywords

  • Chromosomal aberrations
  • Cytochromes P450
  • Kaempferol
  • Mutagenicity
  • Quercetin
  • V79 cells

Cite this

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title = "Mutagenicity of kaempferol in V79 cells: The role of cytochromes P450",
abstract = "The flavonol kaempferol is widely found in the diet and is directly mutagenic in some short-term tests, such as the induction of chromosomal aberrations in eukaryotic cells. The presence of exogenous metabolizing systems enhances its mutagenicity. We have evaluated the role of cytochromes P450 in the induction of chromosomal aberrations by kaempferol in V79 cells. The results obtained suggest that there is a time-dependent biotransformation of kaempferol to quercetin, by cytochromes P450, as assessed by high pressure liquid chromatography. Quercetin seems to contribute to the mutagenicity of kaempferol in the presence of microsomal metabolizing systems. On the other hand, the direct induction of chromosomal aberrations by kaempferol does not seem to depend on the production of reactive oxygen species.",
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}

Mutagenicity of kaempferol in V79 cells : The role of cytochromes P450. / Duarte Silva, I.; Rodrigues, A.; Gaspar, J.; Maia, R.; Laires, A.; Rueff, José.

In: Teratogenesis Carcinogenesis And Mutagenesis, Vol. 16, No. 4, 1996, p. 229-241.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Mutagenicity of kaempferol in V79 cells

T2 - The role of cytochromes P450

AU - Duarte Silva, I.

AU - Rodrigues, A.

AU - Gaspar, J.

AU - Maia, R.

AU - Laires, A.

AU - Rueff, José

PY - 1996

Y1 - 1996

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AB - The flavonol kaempferol is widely found in the diet and is directly mutagenic in some short-term tests, such as the induction of chromosomal aberrations in eukaryotic cells. The presence of exogenous metabolizing systems enhances its mutagenicity. We have evaluated the role of cytochromes P450 in the induction of chromosomal aberrations by kaempferol in V79 cells. The results obtained suggest that there is a time-dependent biotransformation of kaempferol to quercetin, by cytochromes P450, as assessed by high pressure liquid chromatography. Quercetin seems to contribute to the mutagenicity of kaempferol in the presence of microsomal metabolizing systems. On the other hand, the direct induction of chromosomal aberrations by kaempferol does not seem to depend on the production of reactive oxygen species.

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KW - Cytochromes P450

KW - Kaempferol

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JO - Teratogenesis Carcinogenesis And Mutagenesis

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