Multiple Phlebovirus (Bunyaviridae) genetic groups detected in Rhipicephalus, Hyalomma and Dermacentor ticks from southern Portugal

André Pereira, Lúcia Figueira, Mónica Nunes, Aida Esteves, António José Cotão, Maria Luísa Vieira, Carla Maia, Lenea Campino, Ricardo Parreira

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In the last decade, various research groups have reported a large diversity of new tick-borne phleboviruses, mostly prompted by the discovery of important human pathogens such as the Heartland and severe fever with thrombocytopenia syndrome viruses. Since these analyses have rarely been conducted using ticks collected from Southern Europe, this study was carried out so as to bring new insights into the diversity of phleboviruses circulating in Southern Portugal. Tick specimens were collected from the vegetation (questing ticks) or directly from animals (feeding ticks), and the majority analysed in pools using a detection strategy targeting the large (L) viral genomic segment. A high number of pools revealed the presence of phebovirus sequences, regardless of gender (male/female), origin (questing/feeding) or even species of the tick analysed. These sequences apparently formed three different groups in phylogenetic trees, and encoded L proteins characterized by group-specific amino acid residues. Furthermore, under the conditions used, these viruses failed to replicate in both Vero and DH82 cells. The impact these viruses may have on human/animal health will be addressed in the future.

Original languageEnglish
Pages (from-to)45-52
Number of pages8
JournalTicks and Tick-borne Diseases
Volume8
Issue number1
DOIs
Publication statusPublished - Jan 2017

Fingerprint

Phlebovirus
Bunyaviridae
Dermacentor
Rhipicephalus
Hyalomma
Portugal
Ticks
ticks
Viruses
viruses
Vero Cells
thrombocytopenia
Southern European region
animal feeding
animal health
Thrombocytopenia
fever
Fever
genomics
Amino Acids

Keywords

  • L-segment sequences
  • Phleboviruses
  • Phylogenetic characterization
  • Southern Portugal
  • Ticks

Cite this

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title = "Multiple Phlebovirus (Bunyaviridae) genetic groups detected in Rhipicephalus, Hyalomma and Dermacentor ticks from southern Portugal",
abstract = "In the last decade, various research groups have reported a large diversity of new tick-borne phleboviruses, mostly prompted by the discovery of important human pathogens such as the Heartland and severe fever with thrombocytopenia syndrome viruses. Since these analyses have rarely been conducted using ticks collected from Southern Europe, this study was carried out so as to bring new insights into the diversity of phleboviruses circulating in Southern Portugal. Tick specimens were collected from the vegetation (questing ticks) or directly from animals (feeding ticks), and the majority analysed in pools using a detection strategy targeting the large (L) viral genomic segment. A high number of pools revealed the presence of phebovirus sequences, regardless of gender (male/female), origin (questing/feeding) or even species of the tick analysed. These sequences apparently formed three different groups in phylogenetic trees, and encoded L proteins characterized by group-specific amino acid residues. Furthermore, under the conditions used, these viruses failed to replicate in both Vero and DH82 cells. The impact these viruses may have on human/animal health will be addressed in the future.",
keywords = "L-segment sequences, Phleboviruses, Phylogenetic characterization, Southern Portugal, Ticks",
author = "Andr{\'e} Pereira and L{\'u}cia Figueira and M{\'o}nica Nunes and Aida Esteves and Cot{\~a}o, {Ant{\'o}nio Jos{\'e}} and Vieira, {Maria Lu{\'i}sa} and Carla Maia and Lenea Campino and Ricardo Parreira",
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Multiple Phlebovirus (Bunyaviridae) genetic groups detected in Rhipicephalus, Hyalomma and Dermacentor ticks from southern Portugal. / Pereira, André; Figueira, Lúcia; Nunes, Mónica; Esteves, Aida; Cotão, António José; Vieira, Maria Luísa; Maia, Carla; Campino, Lenea; Parreira, Ricardo.

In: Ticks and Tick-borne Diseases, Vol. 8, No. 1, 01.2017, p. 45-52.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Multiple Phlebovirus (Bunyaviridae) genetic groups detected in Rhipicephalus, Hyalomma and Dermacentor ticks from southern Portugal

AU - Pereira, André

AU - Figueira, Lúcia

AU - Nunes, Mónica

AU - Esteves, Aida

AU - Cotão, António José

AU - Vieira, Maria Luísa

AU - Maia, Carla

AU - Campino, Lenea

AU - Parreira, Ricardo

N1 - Copyright © 2016 Elsevier GmbH. All rights reserved.

PY - 2017/1

Y1 - 2017/1

N2 - In the last decade, various research groups have reported a large diversity of new tick-borne phleboviruses, mostly prompted by the discovery of important human pathogens such as the Heartland and severe fever with thrombocytopenia syndrome viruses. Since these analyses have rarely been conducted using ticks collected from Southern Europe, this study was carried out so as to bring new insights into the diversity of phleboviruses circulating in Southern Portugal. Tick specimens were collected from the vegetation (questing ticks) or directly from animals (feeding ticks), and the majority analysed in pools using a detection strategy targeting the large (L) viral genomic segment. A high number of pools revealed the presence of phebovirus sequences, regardless of gender (male/female), origin (questing/feeding) or even species of the tick analysed. These sequences apparently formed three different groups in phylogenetic trees, and encoded L proteins characterized by group-specific amino acid residues. Furthermore, under the conditions used, these viruses failed to replicate in both Vero and DH82 cells. The impact these viruses may have on human/animal health will be addressed in the future.

AB - In the last decade, various research groups have reported a large diversity of new tick-borne phleboviruses, mostly prompted by the discovery of important human pathogens such as the Heartland and severe fever with thrombocytopenia syndrome viruses. Since these analyses have rarely been conducted using ticks collected from Southern Europe, this study was carried out so as to bring new insights into the diversity of phleboviruses circulating in Southern Portugal. Tick specimens were collected from the vegetation (questing ticks) or directly from animals (feeding ticks), and the majority analysed in pools using a detection strategy targeting the large (L) viral genomic segment. A high number of pools revealed the presence of phebovirus sequences, regardless of gender (male/female), origin (questing/feeding) or even species of the tick analysed. These sequences apparently formed three different groups in phylogenetic trees, and encoded L proteins characterized by group-specific amino acid residues. Furthermore, under the conditions used, these viruses failed to replicate in both Vero and DH82 cells. The impact these viruses may have on human/animal health will be addressed in the future.

KW - L-segment sequences

KW - Phleboviruses

KW - Phylogenetic characterization

KW - Southern Portugal

KW - Ticks

U2 - 10.1016/j.ttbdis.2016.09.015

DO - 10.1016/j.ttbdis.2016.09.015

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EP - 52

JO - Ticks and Tick-borne Diseases

JF - Ticks and Tick-borne Diseases

SN - 1877-959X

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ER -