Multiple origins of knockdown resistance mutations in the afrotropical mosquito vector Anopheles gambiae

João Pinto, Amy Lynd, José L. Vicente, Federica Santolamazza, Nadine P. Randle, Gabriele Gentile, Marta Moreno, Frédéric Simard, Jacques Derek Charlwood, Virgilio Estólio do Rosário, Adalgisa Caccone, Alessandra Della Torre, Martin J. Donnelly

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

How often insecticide resistance mutations arise in natural insect populations is a fundamental question for understanding the evolution of resistance and also for modeling its spread. Moreover, the development of resistance is regarded as a favored model to study the molecular evolution of adaptive traits. In the malaria vector Anopheles gambiae two point mutations (L1014F and L1014S) in the voltage-gated sodium channel gene, that confer knockdown resistance (kdr) to DDT and pyrethroid insecticides, have been described. In order to determine whether resistance alleles result from single or multiple mutation events, genotyping of the kdr locus and partial sequencing of the upstream intron-1 was performed on a total of 288 A. gambiae S-form collected from 28 localities in 15 countries. Knockdown resistance alleles were found to be widespread in West Africa with co-occurrence of both 1014S and 1014F in West-Central localities. Differences in intron-1 haplotype composition suggest that kdr alleles may have arisen from at least four independent mutation events. Neutrality tests provided evidence for a selective sweep acting on this genomic region, particularly in West Africa. The frequency and distribution of these kdr haplotypes varied geographically, being influenced by an interplay between different mutational occurrences, gene flow and local selection. This has important practical implications for the management and sustainability of malaria vector control programs.

Original languageEnglish
Article numbere1243
Number of pages7
JournalPlosOne
Volume2
Issue number11
DOIs
Publication statusPublished - 28 Nov 2007

UN Sustainable Development Goals (SDGs)

  • SDG 3 - Good Health and Well-Being
  • SDG 15 - Life on Land

Fingerprint Dive into the research topics of 'Multiple origins of knockdown resistance mutations in the afrotropical mosquito vector Anopheles gambiae'. Together they form a unique fingerprint.

Cite this