Multidrug-Resistant Iberian Epidemic Clone of Methicillin-Resistant Staphylococcus aureus Endemic in a Hospital in Northern Portugal

I. Santos Sanches, M. Aires de Sousa, L. Sobral, I. Calheiros, L. Felicio, I. Pedra, H. de Lencastre

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Forty-two methicillin-resistant Staphylococcus aureus (MRSA) isolates collected during 1992–1995 at a hospital in the north of Portugal were characterized by a variety of genomic fingerprints. Hybridization of ClaI and SmaI restriction digests with the mecA- and Tn554-specific DNA probes was used to identify polymorphs and determine their localization in chromosomal DNA preparations, and pulsed-field gel electrophoretic analysis of SmaI digests was used to determine chromosomal backgrounds. A major clone (and its variants) carrying the mecA polymorph I, Tn554 type E in the PFGE background of pattern A, accounted for 85% of all MRSA tested in 1992–1993 and 66% in 1994–1995. This clone is closely related to the epidemic Iberian clone that was associated with outbreaks in Spain during 1989–1993 and was endemic in 1992–1993 in two hospitals in Lisbon (Portugal).

Original languageEnglish
Pages (from-to)299-306
Number of pages8
JournalMicrobial Drug Resistance
Volume1
Issue number4
DOIs
Publication statusPublished - 1 Jan 1995

Fingerprint Dive into the research topics of 'Multidrug-Resistant Iberian Epidemic Clone of Methicillin-Resistant Staphylococcus aureus Endemic in a Hospital in Northern Portugal'. Together they form a unique fingerprint.

Cite this