Abstract

Cell shape, size and self-motility appear as determinant intrinsic cell factors in the rheological behavior of living bacterial cultures during the growth process. In this work three different species were considered due to their differences on these intrinsic characteristics: two different strains of Staphylococcus aureus - strain COL and its isogenic cell wall autolysis mutant, RUSAL9 - both non-motile and Escherichia coli and Bacillus subtilis - both presenting intrinsic motility. In situ real-time rheology, was used to characterize the activity of growing bacteria, under steady-shear conditions, in particular the viscosity growth curve was measured, for a constant shear flow rate, presenting for all studied cultures, different and rich flow curves. These complex rheological behaviors are a consequence of two coupled effects: the cell density continuous increase and its changing interacting properties, where cell size and shape and intrinsic motility are major players.

Original languageEnglish
Pages (from-to)26
JournalEuropean Physical Journal E
Volume42
Issue number3
DOIs
Publication statusPublished - 1 Mar 2019

Fingerprint

Cell Shape
Rheology
Cell Size
Bacteria
Autolysis
Intrinsic Factor
Growth
Bacillus subtilis
Viscosity
Cell Wall
Staphylococcus aureus
Cell Count
Escherichia coli

Keywords

  • Topical issue
  • Flowing Matter
  • Problems and Applications

Cite this

@article{d1489757252640258c51c1279192b501,
title = "Motility and cell shape roles in the rheology of growing bacteria cultures",
abstract = "Cell shape, size and self-motility appear as determinant intrinsic cell factors in the rheological behavior of living bacterial cultures during the growth process. In this work three different species were considered due to their differences on these intrinsic characteristics: two different strains of Staphylococcus aureus - strain COL and its isogenic cell wall autolysis mutant, RUSAL9 - both non-motile and Escherichia coli and Bacillus subtilis - both presenting intrinsic motility. In situ real-time rheology, was used to characterize the activity of growing bacteria, under steady-shear conditions, in particular the viscosity growth curve was measured, for a constant shear flow rate, presenting for all studied cultures, different and rich flow curves. These complex rheological behaviors are a consequence of two coupled effects: the cell density continuous increase and its changing interacting properties, where cell size and shape and intrinsic motility are major players.",
keywords = "Topical issue, Flowing Matter, Problems and Applications",
author = "R. Portela and Almeida, {P. L.} and Sobral, {R. G.} and Leal, {C R}",
note = "info:eu-repo/grantAgreement/FCT/5876/147333/PT# info:eu-repo/grantAgreement/FCT/5876/147258/PT# This article is based upon work from COST Action MP1305, supported by COST (European Cooperation in Science and Technology). This work was also supported by FEDER through COMPETE 2020; FCT Projects No. UID/CTM/50025/2013 and PTDC/FIS-NAN/0117/2014 (PLA); ESCMID Grant No. 2015 (RGS); and the Unidade de Ciencias Biomoleculares Aplicadas -UCIBIO, which is financed by national funds from FCT/MEC (Grant No. UID/Multi/04378/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement No. POCI-01-0145-FEDER-007728.",
year = "2019",
month = "3",
day = "1",
doi = "10.1140/epje/i2019-11787-9",
language = "English",
volume = "42",
pages = "26",
journal = "European Physical Journal E",
issn = "1292-8941",
publisher = "Springer Science Business Media",
number = "3",

}

TY - JOUR

T1 - Motility and cell shape roles in the rheology of growing bacteria cultures

AU - Portela, R.

AU - Almeida, P. L.

AU - Sobral, R. G.

AU - Leal, C R

N1 - info:eu-repo/grantAgreement/FCT/5876/147333/PT# info:eu-repo/grantAgreement/FCT/5876/147258/PT# This article is based upon work from COST Action MP1305, supported by COST (European Cooperation in Science and Technology). This work was also supported by FEDER through COMPETE 2020; FCT Projects No. UID/CTM/50025/2013 and PTDC/FIS-NAN/0117/2014 (PLA); ESCMID Grant No. 2015 (RGS); and the Unidade de Ciencias Biomoleculares Aplicadas -UCIBIO, which is financed by national funds from FCT/MEC (Grant No. UID/Multi/04378/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement No. POCI-01-0145-FEDER-007728.

PY - 2019/3/1

Y1 - 2019/3/1

N2 - Cell shape, size and self-motility appear as determinant intrinsic cell factors in the rheological behavior of living bacterial cultures during the growth process. In this work three different species were considered due to their differences on these intrinsic characteristics: two different strains of Staphylococcus aureus - strain COL and its isogenic cell wall autolysis mutant, RUSAL9 - both non-motile and Escherichia coli and Bacillus subtilis - both presenting intrinsic motility. In situ real-time rheology, was used to characterize the activity of growing bacteria, under steady-shear conditions, in particular the viscosity growth curve was measured, for a constant shear flow rate, presenting for all studied cultures, different and rich flow curves. These complex rheological behaviors are a consequence of two coupled effects: the cell density continuous increase and its changing interacting properties, where cell size and shape and intrinsic motility are major players.

AB - Cell shape, size and self-motility appear as determinant intrinsic cell factors in the rheological behavior of living bacterial cultures during the growth process. In this work three different species were considered due to their differences on these intrinsic characteristics: two different strains of Staphylococcus aureus - strain COL and its isogenic cell wall autolysis mutant, RUSAL9 - both non-motile and Escherichia coli and Bacillus subtilis - both presenting intrinsic motility. In situ real-time rheology, was used to characterize the activity of growing bacteria, under steady-shear conditions, in particular the viscosity growth curve was measured, for a constant shear flow rate, presenting for all studied cultures, different and rich flow curves. These complex rheological behaviors are a consequence of two coupled effects: the cell density continuous increase and its changing interacting properties, where cell size and shape and intrinsic motility are major players.

KW - Topical issue

KW - Flowing Matter

KW - Problems and Applications

U2 - 10.1140/epje/i2019-11787-9

DO - 10.1140/epje/i2019-11787-9

M3 - Article

VL - 42

SP - 26

JO - European Physical Journal E

JF - European Physical Journal E

SN - 1292-8941

IS - 3

ER -