TY - JOUR
T1 - Molecularly-imprinted chloramphenicol sensor with laser-induced graphene electrodes
AU - Cardoso, Ana R.
AU - Marques, Ana C.
AU - Santos, Lídia
AU - Carvalho, Alexandre F.
AU - Costa, Florinda M.
AU - Martins, Rodrigo
AU - Sales, M. Goreti F.
AU - Fortunato, Elvira
N1 - info:eu-repo/grantAgreement/FCT/5876/147333/PT#
PTDC/AAG-TEC/5400/2014.
POCI-01-0145-FEDER-016637.
POCI-01-0145-FEDER-007688. Grant SFRH/BD/130107/2017. SFRH/BD/115173/2016 DAEPHYS-FCT PD/BD/114063/2015, "Programa de Estimulo a Investigagao 2016" from Fundacao Calouste Gulbenkian.
Sem PDF conforme despacho.
PY - 2019/1/15
Y1 - 2019/1/15
N2 - Graphene has emerged as a novel material with enhanced electrical and structural properties that can be used for a multitude of applications from super-capacitors to biosensors. In this context, an ultra-sensitive biosensor was developed using a low-cost, simple and mask-free method based on laser-induced graphene technique for electrodes patterning. The graphene was produced on a polyimide substrate, showing a porous multi-layer structure with a resistivity of 102.4 ± 7.3 Ω/square. The biosensor was designed as a 3-electrode system. Auxiliary and working electrodes were made of graphene by laser patterning and the reference electrode was handmade by casting a silver ink. A molecularly-imprinted polymer (MIP) was produced at the working electrode by direct electropolymerization of eriochrome black T (EBT). As proof-of-concept, the MIP film was tailored for chloramphenicol (CAP), a common contaminant in aquaculture. The resulting device was evaluated by cyclic voltammetry and electrochemical impedance spectroscopy readings against a redox standard probe. The limit of detection (LOD) was 0.62 nM and the linear response ranged from 1 nM to 10 mM. These analytical features were better than those produced by assembling the same biorecognition element on commercial graphene- and carbon-based screen-printed electrodes. Overall, the simplicity and quickness of the laser-induced graphene technique, along with the better analytical features obtained with the graphene-based electrodes, shows the potential to become a commercial approach for on-site sensing.
AB - Graphene has emerged as a novel material with enhanced electrical and structural properties that can be used for a multitude of applications from super-capacitors to biosensors. In this context, an ultra-sensitive biosensor was developed using a low-cost, simple and mask-free method based on laser-induced graphene technique for electrodes patterning. The graphene was produced on a polyimide substrate, showing a porous multi-layer structure with a resistivity of 102.4 ± 7.3 Ω/square. The biosensor was designed as a 3-electrode system. Auxiliary and working electrodes were made of graphene by laser patterning and the reference electrode was handmade by casting a silver ink. A molecularly-imprinted polymer (MIP) was produced at the working electrode by direct electropolymerization of eriochrome black T (EBT). As proof-of-concept, the MIP film was tailored for chloramphenicol (CAP), a common contaminant in aquaculture. The resulting device was evaluated by cyclic voltammetry and electrochemical impedance spectroscopy readings against a redox standard probe. The limit of detection (LOD) was 0.62 nM and the linear response ranged from 1 nM to 10 mM. These analytical features were better than those produced by assembling the same biorecognition element on commercial graphene- and carbon-based screen-printed electrodes. Overall, the simplicity and quickness of the laser-induced graphene technique, along with the better analytical features obtained with the graphene-based electrodes, shows the potential to become a commercial approach for on-site sensing.
KW - Chloramphenicol
KW - Laser irradiation
KW - Molecularly-imprinted polymer
KW - On-site graphene production
KW - Three-electrode system
UR - http://www.scopus.com/inward/record.url?scp=85055326581&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2018.10.015
DO - 10.1016/j.bios.2018.10.015
M3 - Article
C2 - 30388558
AN - SCOPUS:85055326581
VL - 124-125
SP - 167
EP - 175
JO - Biosensors & Bioelectronics
JF - Biosensors & Bioelectronics
SN - 0956-5663
ER -