Molecular types of methicillin-resistant Staphylococcus aureus and methicillin-sensitive S. aureus strains causing skin and soft tissue infections and nasal colonization, identified in community health centers in New York City

Maria Pardos De La Gandara, Juan Antonio Raygoza Garay, Michael Mwangi, Jonathan N. Tobin, Amanda Tsang, Chamanara Khalida, Brianna D'Orazio, Rhonda G. Kost, Andrea Leinberger-Jabari, Cameron Coffran, Teresa H. Evering, Barry S. Coller, Shirish Balachandra, Tracie Urban, Claude Parola, Scott Salvato, Nancy Jenks, Daren Wu, Rhonda Burgess, Marilyn ChungHerminia Garcez Lencastre, Alexander Tomasz

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

In November 2011, The Rockefeller University Center for Clinical and Translational Science (CCTS), the Laboratory of Microbiology and Infectious Diseases, and Clinical Directors Network (CDN) launched a research and learning collaborative project with six community health centers in the New York City metropolitan area to determine the nature (clonal type) of community-acquired Staphylococcus aureus strains causing skin and soft tissue infections (SSTIs). Between November 2011 and March 2013, wound and nasal samples from 129 patients with active SSTIs suspicious for S. aureus were collected and characterized by molecular typing techniques. In 63 of 129 patients, the skin wounds were infected by S. aureus: methicillinresistant S. aureus (MRSA) was recovered from 39 wounds and methicillin-sensitive S. aureus (MSSA) was recovered from 24. Most-46 of the 63-wound isolates belonged to the CC8/Panton-Valentine leukocidin-positive (PVL+) group of S. aureus clone USA300: 34 of these strains were MRSA and 12 were MSSA. Of the 63 patients with S. aureus infections, 30 were also colonized by S. aureus in the nares: 16 of the colonizing isolates were MRSA, and 14 were MSSA, and the majority of the colonizing isolates belonged to the USA300 clonal group. In most cases (70%), the colonizing isolate belonged to the same clonal type as the strain involved with the infection. In three of the patients, the identity of invasive and colonizing MRSA isolates was further documented by whole-genome sequencing.

Original languageEnglish
Pages (from-to)2648-2658
Number of pages11
JournalJournal Of Clinical Microbiology
Volume53
Issue number8
DOIs
Publication statusPublished - 1 Aug 2015

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