TY - JOUR
T1 - Molecular types of methicillin-resistant Staphylococcus aureus and methicillin-sensitive S. aureus strains causing skin and soft tissue infections and nasal colonization, identified in community health centers in New York City
AU - De La Gandara, Maria Pardos
AU - Garay, Juan Antonio Raygoza
AU - Mwangi, Michael
AU - Tobin, Jonathan N.
AU - Tsang, Amanda
AU - Khalida, Chamanara
AU - D'Orazio, Brianna
AU - Kost, Rhonda G.
AU - Leinberger-Jabari, Andrea
AU - Coffran, Cameron
AU - Evering, Teresa H.
AU - Coller, Barry S.
AU - Balachandra, Shirish
AU - Urban, Tracie
AU - Parola, Claude
AU - Salvato, Scott
AU - Jenks, Nancy
AU - Wu, Daren
AU - Burgess, Rhonda
AU - Chung, Marilyn
AU - Lencastre, Herminia Garcez
AU - Tomasz, Alexander
PY - 2015/8/1
Y1 - 2015/8/1
N2 - In November 2011, The Rockefeller University Center for Clinical and Translational Science (CCTS), the Laboratory of Microbiology and Infectious Diseases, and Clinical Directors Network (CDN) launched a research and learning collaborative project with six community health centers in the New York City metropolitan area to determine the nature (clonal type) of community-acquired Staphylococcus aureus strains causing skin and soft tissue infections (SSTIs). Between November 2011 and March 2013, wound and nasal samples from 129 patients with active SSTIs suspicious for S. aureus were collected and characterized by molecular typing techniques. In 63 of 129 patients, the skin wounds were infected by S. aureus: methicillinresistant S. aureus (MRSA) was recovered from 39 wounds and methicillin-sensitive S. aureus (MSSA) was recovered from 24. Most-46 of the 63-wound isolates belonged to the CC8/Panton-Valentine leukocidin-positive (PVL+) group of S. aureus clone USA300: 34 of these strains were MRSA and 12 were MSSA. Of the 63 patients with S. aureus infections, 30 were also colonized by S. aureus in the nares: 16 of the colonizing isolates were MRSA, and 14 were MSSA, and the majority of the colonizing isolates belonged to the USA300 clonal group. In most cases (70%), the colonizing isolate belonged to the same clonal type as the strain involved with the infection. In three of the patients, the identity of invasive and colonizing MRSA isolates was further documented by whole-genome sequencing.
AB - In November 2011, The Rockefeller University Center for Clinical and Translational Science (CCTS), the Laboratory of Microbiology and Infectious Diseases, and Clinical Directors Network (CDN) launched a research and learning collaborative project with six community health centers in the New York City metropolitan area to determine the nature (clonal type) of community-acquired Staphylococcus aureus strains causing skin and soft tissue infections (SSTIs). Between November 2011 and March 2013, wound and nasal samples from 129 patients with active SSTIs suspicious for S. aureus were collected and characterized by molecular typing techniques. In 63 of 129 patients, the skin wounds were infected by S. aureus: methicillinresistant S. aureus (MRSA) was recovered from 39 wounds and methicillin-sensitive S. aureus (MSSA) was recovered from 24. Most-46 of the 63-wound isolates belonged to the CC8/Panton-Valentine leukocidin-positive (PVL+) group of S. aureus clone USA300: 34 of these strains were MRSA and 12 were MSSA. Of the 63 patients with S. aureus infections, 30 were also colonized by S. aureus in the nares: 16 of the colonizing isolates were MRSA, and 14 were MSSA, and the majority of the colonizing isolates belonged to the USA300 clonal group. In most cases (70%), the colonizing isolate belonged to the same clonal type as the strain involved with the infection. In three of the patients, the identity of invasive and colonizing MRSA isolates was further documented by whole-genome sequencing.
UR - http://www.scopus.com/inward/record.url?scp=84938149978&partnerID=8YFLogxK
U2 - 10.1128/JCM.00591-15
DO - 10.1128/JCM.00591-15
M3 - Article
AN - SCOPUS:84938149978
SN - 0095-1137
VL - 53
SP - 2648
EP - 2658
JO - Journal Of Clinical Microbiology
JF - Journal Of Clinical Microbiology
IS - 8
ER -