Molecular techniques for dicistrovirus detection without RNA extraction or purification.

Marcelo Silva

doi:10.1155/2013/218593

Molecular techniques for dicistrovirus detection without RNA extraction or purification.

Marcelo Silva

Centro de Malária e outras Doenças Tropicais (CMDT)

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

1 Downloads (Pure)

Abstract

Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines. The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus.

Original language	Unknown
Pages (from-to)	218593
Journal	BioMed Research International
Volume	2013
Issue number	NA
DOIs	https://doi.org/10.1155/2013/218593
Publication status	Published - 1 Jan 2013

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1155/2013/218593Licence: CC BY

Molecular Techniques for Dicistrovirus DetectionFinal published version, 1.26 MBLicence: CC BY

Cite this

@article{0cb3e4d57bf14502af6943f18c309692,

title = "Molecular techniques for dicistrovirus detection without RNA extraction or purification.",

abstract = "Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines. The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus.",

author = "Marcelo Silva",

year = "2013",

month = jan,

day = "1",

doi = "10.1155/2013/218593",

language = "Unknown",

volume = "2013",

pages = "218593",

journal = "BioMed Research International",

issn = "2314-6133",

publisher = "Hindawi",

number = "NA",

}

TY - JOUR

T1 - Molecular techniques for dicistrovirus detection without RNA extraction or purification.

AU - Silva, Marcelo

PY - 2013/1/1

Y1 - 2013/1/1

N2 - Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines. The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus.

AB - Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines. The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus.

U2 - 10.1155/2013/218593

DO - 10.1155/2013/218593

M3 - Article

C2 - 23710438

VL - 2013

SP - 218593

JO - BioMed Research International

JF - BioMed Research International

SN - 2314-6133

IS - NA

ER -