Molecular techniques for dicistrovirus detection without RNA extraction or purification.

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Abstract

Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines. The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus.
Original languageUnknown
Pages (from-to)218593
JournalBioMed Research International
Volume2013
Issue numberNA
DOIs
Publication statusPublished - 1 Jan 2013

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