Molecular Recognition of a Thomsen–Friedenreich Antigen Mimetic Targeting Human Galectin-3

Sabrina Santarsia, Ana Sofia Grosso, Filipa Trovão, Jesús Jiménez-Barbero, Ana Luísa Carvalho, Cristina Nativi, Filipa Marcelo

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Overexpression of the Thomsen–Friedenreich (TF) antigen in cell membrane proteins occurs in 90 % of adenocarcinomas. Additionally, the binding of the TF antigen to human galectin-3 (Gal-3), also frequently overexpressed in malignancy, promotes cancer progression and metastasis. In this context, structures that interfere with this specific interaction have the potential to prevent cancer metastasis. A multidisciplinary approach combining the optimized synthesis of a TF antigen mimetic with NMR, X-ray crystallography methods, and isothermal titration calorimetry assays was used to unravel the molecular structural details that govern the Gal-3/TF mimetic interaction. The TF mimetic has a binding affinity for Gal-3 similar to that of the TF natural antigen and retains the binding epitope and bioactive conformation observed for the native antigen. Furthermore, from a thermodynamic perspective, a decrease in the enthalpic contribution was observed for the Gal-3/TF mimetic complex; however, this behavior is compensated by a favorable gain in entropy. From a structural perspective, these results establish our TF mimetic as a scaffold to design multivalent solutions to potentially interfere with Gal-3 aberrant interactions and for likely use in hampering Gal-3-mediated cancer cell adhesion and metastasis.

Original languageEnglish
Pages (from-to)2030-2036
Number of pages7
JournalChemmedchem
Volume13
Issue number19
DOIs
Publication statusPublished - 8 Oct 2018

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Keywords

  • galectin-3
  • molecular recognition
  • NMR spectroscopy
  • tumor-associated carbohydrate antigens
  • X-ray crystallography

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