Molecular cloning and sequence analysis of the gene of the molybdenum‐containing aldehyde oxido‐reductase of Desulfovibrio gigas The deduced amino acid sequence shows similarity to xanthine dehydrogenase

Ulrich Thoenes, Orfeu L. Flores, Ana Neves, Bart Devreese, Jozef J. van Beeumen, Robert Huber, Maria J. Romão, Jean LeGall, José J.G. Moura, Claudina Rodrigues‐Pousada

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Abstract

In this report, we describe the isolation of a 4020‐bp genomic PstI fragment of Desulfovibrio gigas harboring the aldehyde oxido‐reductase gene. The aldehyde oxido‐reductase gene spans 2718 bp of genomic DNA and codes for a protein with 906 residues. The protein sequence shows an average 52% (± 1.5%) similarity to xanthine dehydrogenase from different organisms. The codon usage of the aldehyde oxidoreductase is almost identical to a calculated codon usage of the Desulfovibrio bacteria.

Original languageEnglish
Pages (from-to)901-910
Number of pages10
JournalEuropean Journal Of Biochemistry
Volume220
Issue number3
DOIs
Publication statusPublished - 1 Jan 1994

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