TY - JOUR
T1 - Molecular characterisation of Cryptosporidium isolates from pre-weaned calves in Romania
T2 - Is there an actual risk of zoonotic infections?
AU - Imre, Kálmán
AU - Lobo, ML
AU - Matos, Olga
AU - Popescu, Cristina
AU - Genchi, Claudio
AU - Dǎrǎbuş, Gheorghe
PY - 2011/9/27
Y1 - 2011/9/27
N2 - Diarrheic fecal samples from 258 pre-weaned calves (1-30 day-old) from 9 dairy farms located in Banat region, Romania, were microscopically examined for the presence of Cryptosporidium oocysts. Overall, 65 (25%) samples were found positive. A higher percent of infection was recorded in calves aged between 8 and 14 days compared with other age categories (1-7, 8-14, 15-21 and 22-30 days; p<0.05). Genetic characterization was carried out on all Cryptosporidium-positive samples. After DNA extraction, Cryptosporidium species were determined by a nested PCR of the small subunit rRNA gene (18S) followed by RFLP analysis with SspI, VspI and MboII restriction enzymes. The restriction patterns showed that animals were infected with Cryptosporidium parvum. Subsequently, subtyping of 13 C. parvum isolates, based on sequence analysis of the 60. kDa glycoprotein (GP60) gene, showed 2 subtypes (IIaA15G2R1 and IIaA16G1R1) belonging to the subtype family IIa. This is the first molecular study of bovine Cryptosporidium infection in Romania.
AB - Diarrheic fecal samples from 258 pre-weaned calves (1-30 day-old) from 9 dairy farms located in Banat region, Romania, were microscopically examined for the presence of Cryptosporidium oocysts. Overall, 65 (25%) samples were found positive. A higher percent of infection was recorded in calves aged between 8 and 14 days compared with other age categories (1-7, 8-14, 15-21 and 22-30 days; p<0.05). Genetic characterization was carried out on all Cryptosporidium-positive samples. After DNA extraction, Cryptosporidium species were determined by a nested PCR of the small subunit rRNA gene (18S) followed by RFLP analysis with SspI, VspI and MboII restriction enzymes. The restriction patterns showed that animals were infected with Cryptosporidium parvum. Subsequently, subtyping of 13 C. parvum isolates, based on sequence analysis of the 60. kDa glycoprotein (GP60) gene, showed 2 subtypes (IIaA15G2R1 and IIaA16G1R1) belonging to the subtype family IIa. This is the first molecular study of bovine Cryptosporidium infection in Romania.
KW - Cryptosporidium
KW - GP60 subtypes
KW - PCR-RFLP
KW - Pre-weaned calves
KW - SSU rRNA
UR - http://www.scopus.com/inward/record.url?scp=80052528863&partnerID=8YFLogxK
UR - https://www.sciencedirect.com/science/article/abs/pii/S0304401711003037?via%3Dihub
U2 - 10.1016/j.vetpar.2011.04.042
DO - 10.1016/j.vetpar.2011.04.042
M3 - Article
C2 - 21612871
AN - SCOPUS:80052528863
VL - 181
SP - 321
EP - 324
JO - Veterinary Parasitology
JF - Veterinary Parasitology
SN - 0304-4017
IS - 2-4
ER -
