Molecular Beacon for Detection miRNA-21 as a Biomarker of Lung Cancer

Daniela Alexandre, Bernardo Teixeira, André Rico, Salete Valente, Ana Craveiro, Pedro V. Baptista, Carla Cruz

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)
87 Downloads (Pure)

Abstract

Lung cancer (LC) is the leading cause of cancer-related death worldwide. Although the diagnosis and treatment of non-small cell lung cancer (NSCLC), which accounts for approximately 80% of LC cases, have greatly improved in the past decade, there is still an urgent need to find more sensitive and specific screening methods. Recently, new molecular biomarkers are emerging as potential non-invasive diagnostic agents to screen NSCLC, including multiple microRNAs (miRNAs) that show an unusual expression profile. Moreover, peripheral blood mononuclear cells’ (PBMCs) miRNA profile could be linked with NSCLC and used for diagnosis. We developed a molecular beacon (MB)-based miRNA detection strategy for NSCLC. Following PBMCs isolation and screening of the expression profile of a panel of miRNA by RT-qPCR, we designed a MB targeting of up-regulated miR-21-5p. This MB 21-5p was characterized by FRET-melting, CD, NMR and native PAGE, allowing the optimization of an in-situ approach involving miR-21-5p detection in PBMCs via MB. Data show the developed MB approach potential for miR-21-5p detection in PBMCs from clinical samples towards NSCLC.

Original languageEnglish
Article number3330
Number of pages14
JournalInternational Journal of Molecular Sciences
Volume23
Issue number6
DOIs
Publication statusPublished - 19 Mar 2022

Keywords

  • Diagnosis
  • Lung cancer
  • MicroRNAs
  • Molecular beacon
  • Peripheral blood mononuclear cells

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