Modulation of phase separation at the micron scale and nanoscale in giant polymer/lipid hybrid unilamellar vesicles (GHUVs)

Thi Phuong Tuyen Dao, Fabio Fernandes, Emmanuel Ibarboure, Khalid Ferji, Manuel Prieto, Olivier Sandre, Jean François Le Meins

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Phase separation in giant polymer/lipid hybrid unilamellar vesicles (GHUVs) has been described over the last few years. However there is still a lack of understanding on the physical and molecular factors governing the phase separation in such systems. Among these parameters it has been suggested that in analogy to multicomponent lipid vesicles hydrophobic mismatches as well as lipid fluidity play a role. In this work, we aim to map a global picture of phase separation and domain formation in the membrane of GHUVs by using various copolymers based on poly(dimethylsiloxane) (PDMS) and poly(ethylene glycol) (PEO) with different architectures (grafted, triblock) and molar masses, combined with phospholipids in the fluid (POPC) or gel state (DPPC) at room temperature. From confocal imaging and fluorescence lifetime imaging microscopy (FLIM) techniques, the phase separation into either micro- or nano-domains within GHUVs was studied. In particular, our systematic studies demonstrate that in addition to the lipid/polymer fraction or the lipid physical state, important factors such as line tension at lipid polymer/lipid boundaries can be finely modulated by the molar mass and the architecture of the copolymer and lead to the formation of stable lipid domains with different sizes and morphologies in such GHUVs.

Original languageEnglish
Pages (from-to)627-637
Number of pages11
JournalSoft Matter
Volume13
Issue number3
DOIs
Publication statusPublished - 21 Jan 2017

Keywords

  • MIXED LIPID/POLYMER MEMBRANES
  • INTRAMEMBRANE DOMAINS
  • BLOCK-COPOLYMERS
  • LIPID-MEMBRANES
  • ENERGY-TRANSFER
  • TENSION
  • ORGANIZATION
  • RECOGNITION
  • MORPHOLOGY
  • STABILITY

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