Modulating the protein content of complex proteomes using acetonitrile

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Abstract

In this work we present acetonitrile as a tool to modulate the dynamic range of the proteome of complex samples. Different concentrations of acetonitrile ranging from 15% v/v to 65% v/v were used to modulate the protein content of serum samples from healthy people and patients with lymphoma and myeloma. We show that the proteome above 70 kDa is pelleted as a function of the concentration of acetonitrile and that profiling with PCA or Clustering is only possible using the supernatants obtained for concentrations of acetonitrile higher than 45% v/v or the pellets for concentrations of acetonitrile of 35% and 45%. The differentiation and classification of the three groups of sera samples (healthy, lymphoma and myeloma) were possible using acetonitrile at 55% v/v concentration. This work opens new avenues for the application of acetonitrile as a cost-effective tool in proteomics applications.

Original languageEnglish
Pages (from-to)333-339
Number of pages7
JournalTalanta
Volume182
DOIs
Publication statusPublished - 15 May 2018

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Keywords

  • Acetonitrile
  • Biomarker
  • Cancer
  • Disease
  • Method
  • Sample treatment
  • Serum

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