Abstract

Nanotechnology based diagnostics has provided improved tools for pathogen detection and sensitive and specific characterization of antibiotic resistance signatures. Tuberculosis (TB) is caused by members of the Mycobacterium tuberculosis Complex (MTBC) and, according to the World Health Organization, is one of the most serious infectious diseases in the world. Recent advances in molecular diagnostics of TB have improved both the detection time and sensitivity but they still require specialized technical personnel and cumbersome laboratory equipment. Diagnostics at point-of-need is crucial to TB control as it may provide rapid identification of pathogen together with the resistance profile of TB strains, originated from single nucleotide polymorphisms (SNPs) in different loci , allowing for a more accurate indication of the adequate therapy.Gold nanoparticles have been widely used in molecular diagnostics platforms. Here, we describe the use of gold nanoprobes (oligonucleotide functionalized gold nanoparticles) to be used in a non-crosslinking colorimetric method for the direct detection of specific DNA targets. Due to the remarkable optical properties of gold nanoparticles, this detection system provides colorimetric detection of the pathogen together with the potential of identification of several single nucleotide polymorphisms (SNPs) involved in TB resistance to antibiotics. For point-of-need use, we adapted this strategy to a low-cost mobile scheme using a paper based revelation platform and where the spectral signature is transposed to RGB data via a smartphone device. This way, identification of pathogen and characterization of resistance signatures is achieved at point-of-need.

Original languageEnglish
Title of host publicationMobile Health Technologies: Methods and Protocols
EditorsAvraham Rasooly, Keith E. Herold
Place of PublicationNew York
PublisherHumana Press
Pages41-56
Number of pages16
VolumePart 1
ISBN (Electronic)978-1-4939-2172-0
ISBN (Print)978-1-4939-2171-3
DOIs
Publication statusPublished - 27 Jan 2015

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume1256
ISSN (Print)1064-3745

Fingerprint

Nanoprobes
Pathogens
Gold
Tuberculosis
Nanoparticles
Polymorphism
Molecular Pathology
Microbial Drug Resistance
Nucleotides
Single Nucleotide Polymorphism
Anti-Bacterial Agents
Smartphones
Laboratory Personnel
Equipment and Supplies
Nanotechnology
Oligonucleotides
Mycobacterium tuberculosis
Optical properties
Health
Personnel

Keywords

  • Antibiotic resistance
  • Colorimetric method
  • Gold nanoparticles
  • Mycobacterium tuberculosis
  • Nanoprobes
  • Paper-based diagnostics
  • Point-of-need detection

Cite this

Veigas, B., Fortunato, E., & Baptista, P. V. (2015). Mobile based gold nanoprobe TB diagnostics for point-of-need. In A. Rasooly, & K. E. Herold (Eds.), Mobile Health Technologies: Methods and Protocols (Vol. Part 1, pp. 41-56). (Methods in Molecular Biology; Vol. 1256). New York: Humana Press. https://doi.org/10.1007/978-1-4939-2172-0_3
Veigas, B. ; Fortunato, E. ; Baptista, P. V. / Mobile based gold nanoprobe TB diagnostics for point-of-need. Mobile Health Technologies: Methods and Protocols. editor / Avraham Rasooly ; Keith E. Herold. Vol. Part 1 New York : Humana Press, 2015. pp. 41-56 (Methods in Molecular Biology).
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Veigas, B, Fortunato, E & Baptista, PV 2015, Mobile based gold nanoprobe TB diagnostics for point-of-need. in A Rasooly & KE Herold (eds), Mobile Health Technologies: Methods and Protocols. vol. Part 1, Methods in Molecular Biology, vol. 1256, Humana Press, New York, pp. 41-56. https://doi.org/10.1007/978-1-4939-2172-0_3

Mobile based gold nanoprobe TB diagnostics for point-of-need. / Veigas, B.; Fortunato, E.; Baptista, P. V.

Mobile Health Technologies: Methods and Protocols. ed. / Avraham Rasooly; Keith E. Herold. Vol. Part 1 New York : Humana Press, 2015. p. 41-56 (Methods in Molecular Biology; Vol. 1256).

Research output: Chapter in Book/Report/Conference proceedingChapter

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T1 - Mobile based gold nanoprobe TB diagnostics for point-of-need

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AU - Fortunato, E.

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N2 - Nanotechnology based diagnostics has provided improved tools for pathogen detection and sensitive and specific characterization of antibiotic resistance signatures. Tuberculosis (TB) is caused by members of the Mycobacterium tuberculosis Complex (MTBC) and, according to the World Health Organization, is one of the most serious infectious diseases in the world. Recent advances in molecular diagnostics of TB have improved both the detection time and sensitivity but they still require specialized technical personnel and cumbersome laboratory equipment. Diagnostics at point-of-need is crucial to TB control as it may provide rapid identification of pathogen together with the resistance profile of TB strains, originated from single nucleotide polymorphisms (SNPs) in different loci , allowing for a more accurate indication of the adequate therapy.Gold nanoparticles have been widely used in molecular diagnostics platforms. Here, we describe the use of gold nanoprobes (oligonucleotide functionalized gold nanoparticles) to be used in a non-crosslinking colorimetric method for the direct detection of specific DNA targets. Due to the remarkable optical properties of gold nanoparticles, this detection system provides colorimetric detection of the pathogen together with the potential of identification of several single nucleotide polymorphisms (SNPs) involved in TB resistance to antibiotics. For point-of-need use, we adapted this strategy to a low-cost mobile scheme using a paper based revelation platform and where the spectral signature is transposed to RGB data via a smartphone device. This way, identification of pathogen and characterization of resistance signatures is achieved at point-of-need.

AB - Nanotechnology based diagnostics has provided improved tools for pathogen detection and sensitive and specific characterization of antibiotic resistance signatures. Tuberculosis (TB) is caused by members of the Mycobacterium tuberculosis Complex (MTBC) and, according to the World Health Organization, is one of the most serious infectious diseases in the world. Recent advances in molecular diagnostics of TB have improved both the detection time and sensitivity but they still require specialized technical personnel and cumbersome laboratory equipment. Diagnostics at point-of-need is crucial to TB control as it may provide rapid identification of pathogen together with the resistance profile of TB strains, originated from single nucleotide polymorphisms (SNPs) in different loci , allowing for a more accurate indication of the adequate therapy.Gold nanoparticles have been widely used in molecular diagnostics platforms. Here, we describe the use of gold nanoprobes (oligonucleotide functionalized gold nanoparticles) to be used in a non-crosslinking colorimetric method for the direct detection of specific DNA targets. Due to the remarkable optical properties of gold nanoparticles, this detection system provides colorimetric detection of the pathogen together with the potential of identification of several single nucleotide polymorphisms (SNPs) involved in TB resistance to antibiotics. For point-of-need use, we adapted this strategy to a low-cost mobile scheme using a paper based revelation platform and where the spectral signature is transposed to RGB data via a smartphone device. This way, identification of pathogen and characterization of resistance signatures is achieved at point-of-need.

KW - Antibiotic resistance

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KW - Nanoprobes

KW - Paper-based diagnostics

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SN - 978-1-4939-2171-3

VL - Part 1

T3 - Methods in Molecular Biology

SP - 41

EP - 56

BT - Mobile Health Technologies: Methods and Protocols

A2 - Rasooly, Avraham

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PB - Humana Press

CY - New York

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Veigas B, Fortunato E, Baptista PV. Mobile based gold nanoprobe TB diagnostics for point-of-need. In Rasooly A, Herold KE, editors, Mobile Health Technologies: Methods and Protocols. Vol. Part 1. New York: Humana Press. 2015. p. 41-56. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-2172-0_3