Mixing Block Copolymers with Phospholipids at the Nanoscale

From Hybrid Polymer/Lipid Wormlike Micelles to Vesicles Presenting Lipid Nanodomains

T. P Tuyen Dao, A. Brûlet, F. Fernandes, M. Er-Rafik, Khalid Ferji, R. Schweins, J.-P. Chapel, Alexander Fedorov, M. Schmutz, Manuel Prieto, O. Sandre, Jean François Le Meins

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Hybrids, i.e., intimately mixed polymer/phospholipid vesicles, can potentially marry in a single membrane the best characteristics of the two separate components. The ability of amphiphilic copolymers and phospholipids to self assemble into hybrid membranes has been studied until now on the submicrometer scale using optical microscopy on giant hybrid unilamellar vesicles (GHUVs), but limited information is available on large hybrid unilamellar vesicles (LHUVs). In this work, copolymers based on poly(dimethylsiloxane) and poly (ethylene oxide) with different molar masses and architectures (graft, triblock) were associated with 1,2-dipalmitoyl-sn-glycero3-phosphocholine (DPPC). Classical protocols of LUV formation were used to obtain nanosized self-assembled structures. Using small-angle neutron scattering (SANS), time-resolved Forster resonance energy transfer (TR-FRET), and cryo-transmission electron microscopy (cryo-TEM), we show that copolymer architecture and molar mass have direct influences on the formation of hybrid nanostructures that can range from wormlike hybrid micelles to hybrid vesicles presenting small lipid nanodomains.
Original languageEnglish
Pages (from-to) 1705-1715
JournalLangmuir
Volume33
Issue number7
DOIs
Publication statusPublished - 27 Jan 2017

Fingerprint

Micelles
Block copolymers
Phospholipids
Unilamellar Liposomes
Polymers
Copolymers
Molar mass
Lipids
Membranes
Phosphorylcholine
Neutron scattering
Polyethylene oxides
Grafts
Optical microscopy
Nanostructures
Transmission electron microscopy

Keywords

  • SMALL-ANGLE SCATTERING
  • PHASE-SEPARATION
  • ENERGY-TRANSFER
  • FORM-FACTOR
  • MEMBRANES

Cite this

Dao, T. P Tuyen ; Brûlet, A. ; Fernandes, F. ; Er-Rafik, M. ; Ferji, Khalid ; Schweins, R. ; Chapel, J.-P. ; Fedorov, Alexander ; Schmutz, M. ; Prieto, Manuel ; Sandre, O. ; Le Meins, Jean François. / Mixing Block Copolymers with Phospholipids at the Nanoscale : From Hybrid Polymer/Lipid Wormlike Micelles to Vesicles Presenting Lipid Nanodomains. In: Langmuir. 2017 ; Vol. 33, No. 7. pp. 1705-1715.
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abstract = "Hybrids, i.e., intimately mixed polymer/phospholipid vesicles, can potentially marry in a single membrane the best characteristics of the two separate components. The ability of amphiphilic copolymers and phospholipids to self assemble into hybrid membranes has been studied until now on the submicrometer scale using optical microscopy on giant hybrid unilamellar vesicles (GHUVs), but limited information is available on large hybrid unilamellar vesicles (LHUVs). In this work, copolymers based on poly(dimethylsiloxane) and poly (ethylene oxide) with different molar masses and architectures (graft, triblock) were associated with 1,2-dipalmitoyl-sn-glycero3-phosphocholine (DPPC). Classical protocols of LUV formation were used to obtain nanosized self-assembled structures. Using small-angle neutron scattering (SANS), time-resolved Forster resonance energy transfer (TR-FRET), and cryo-transmission electron microscopy (cryo-TEM), we show that copolymer architecture and molar mass have direct influences on the formation of hybrid nanostructures that can range from wormlike hybrid micelles to hybrid vesicles presenting small lipid nanodomains.",
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Dao, TPT, Brûlet, A, Fernandes, F, Er-Rafik, M, Ferji, K, Schweins, R, Chapel, J-P, Fedorov, A, Schmutz, M, Prieto, M, Sandre, O & Le Meins, JF 2017, 'Mixing Block Copolymers with Phospholipids at the Nanoscale: From Hybrid Polymer/Lipid Wormlike Micelles to Vesicles Presenting Lipid Nanodomains', Langmuir, vol. 33, no. 7, pp. 1705-1715. https://doi.org/10.1021/acs.langmuir.6b04478

Mixing Block Copolymers with Phospholipids at the Nanoscale : From Hybrid Polymer/Lipid Wormlike Micelles to Vesicles Presenting Lipid Nanodomains. / Dao, T. P Tuyen; Brûlet, A.; Fernandes, F.; Er-Rafik, M.; Ferji, Khalid; Schweins, R.; Chapel, J.-P.; Fedorov, Alexander; Schmutz, M.; Prieto, Manuel; Sandre, O.; Le Meins, Jean François.

In: Langmuir, Vol. 33, No. 7, 27.01.2017, p. 1705-1715.

Research output: Contribution to journalArticle

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AU - Dao, T. P Tuyen

AU - Brûlet, A.

AU - Fernandes, F.

AU - Er-Rafik, M.

AU - Ferji, Khalid

AU - Schweins, R.

AU - Chapel, J.-P.

AU - Fedorov, Alexander

AU - Schmutz, M.

AU - Prieto, Manuel

AU - Sandre, O.

AU - Le Meins, Jean François

N1 - Sem pdf conforme despacho. FCT Agence Nationale de la Recherche - SAICTPAC/0019/2015 ; FAPESP/20107/2014 ; UID/NAN/50024/2013 ; KbT 12-BS08-0018-01.

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N2 - Hybrids, i.e., intimately mixed polymer/phospholipid vesicles, can potentially marry in a single membrane the best characteristics of the two separate components. The ability of amphiphilic copolymers and phospholipids to self assemble into hybrid membranes has been studied until now on the submicrometer scale using optical microscopy on giant hybrid unilamellar vesicles (GHUVs), but limited information is available on large hybrid unilamellar vesicles (LHUVs). In this work, copolymers based on poly(dimethylsiloxane) and poly (ethylene oxide) with different molar masses and architectures (graft, triblock) were associated with 1,2-dipalmitoyl-sn-glycero3-phosphocholine (DPPC). Classical protocols of LUV formation were used to obtain nanosized self-assembled structures. Using small-angle neutron scattering (SANS), time-resolved Forster resonance energy transfer (TR-FRET), and cryo-transmission electron microscopy (cryo-TEM), we show that copolymer architecture and molar mass have direct influences on the formation of hybrid nanostructures that can range from wormlike hybrid micelles to hybrid vesicles presenting small lipid nanodomains.

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KW - SMALL-ANGLE SCATTERING

KW - PHASE-SEPARATION

KW - ENERGY-TRANSFER

KW - FORM-FACTOR

KW - MEMBRANES

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DO - 10.1021/acs.langmuir.6b04478

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