Mixed culture polyhydroxyalkanoates production from sugar molasses: The use of a 2-stage CSTR system for culture selection

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Polyhydroxyalkanoates (PHAs) are promising biodegradable polymers The use of mixed microbial cultures (MMC) and low cost feedstocks have a positive impact on the cost-effectiveness of the process It has typically been carried out in Sequencing Batch Reactors (SBR) In this study, a 2-stage CSTR system (under Feast and Famine conditions) was used to effectively select for PHA-storing organisms using fermented molasses as feedstock The effect of influent substrate concentration (60-120 Cmmol VFA/L) and HRT ratio between the reactors (0.2-0.5 h/h) on the system's selection efficiency was assessed. It was shown that Feast reactor residual substrate concentration impacted on the selective pressure for PHA storage (due to substrate-dependent kinetic limitation) Moreover, a residual substrate concentration coming from the Feast to the Famine reactor did not Jeopardize the physiological adaptation required for enhanced PHA storage The culture reached a maximum PHA content of 61% This success opens new perspectives to the use of wastewater treatment infrastructure for PHA production, thus valorizing either excess sludge or wastewaters (C) 2010 Elsevier Ltd. All rights reserved.
Original languageUnknown
Pages (from-to)7112-7122
JournalBioresource Technology
Issue number18
Publication statusPublished - 1 Jan 2010

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