TY - JOUR
T1 - Mining the microbiome of key species from african savanna woodlands
T2 - potential for soil health improvement anwd plant growth promotion
AU - Maquia, Ivete Sandra
AU - Fareleira, Paula
AU - Castro, Isabel Videira E.
AU - Brito, Denise R.A.
AU - Soares, Ricardo
AU - Chaúque, Aniceto
AU - Ferreira-Pinto, M. Manuela
AU - Lumini, Erica
AU - Berruti, Andrea
AU - Ribeiro, Natasha S.
AU - Marques, Isabel
AU - Ribeiro-Barros, Ana I.
N1 - Funding text: This work was supported by funds from Camões, Instituto da Cooperação e da Língua and Fundação para a Ciência e a Tecnologia through the research unit UIDB/00239/2020 (CEF), the PhD grant SFRH/BD/113951/2015 (Ivete Sandra Maquia), and the contribution to the International Rice Research Institute.
PY - 2020/9
Y1 - 2020/9
N2 - (1) Aims: Assessing bacterial diversity and plant-growth-promoting functions in the rhizosphere of the native African trees Colophospermum mopane and Combretum apiculatum in three landscapes of the Limpopo National Park (Mozambique), subjected to two fire regimes. (2) Methods: Bacterial communities were identified through Illumina Miseq sequencing of the 16S rRNA gene amplicons, followed by culture dependent methods to isolate plant growth-promoting bacteria (PGPB). Plant growth-promoting traits of the cultivable bacterial fraction were further analyzed. To screen for the presence of nitrogen-fixing bacteria, the promiscuous tropical legume Vigna unguiculata was used as a trap host. The taxonomy of all purified isolates was genetically verified by 16S rRNA gene Sanger sequencing. (3) Results: Bacterial community results indicated that fire did not drive major changes in bacterial abundance. However, culture-dependent methods allowed the differentiation of bacterial communities between the sampled sites, which were particularly enriched in Proteobacteria with a wide range of plant-beneficial traits, such as plant protection, plant nutrition, and plant growth. Bradyrhizobium was the most frequent symbiotic bacteria trapped in cowpea nodules coexisting with other endophytic bacteria. (4) Conclusion: Although the global analysis did not show significant differences between landscapes or sites with different fire regimes, probably due to the fast recovery of bacterial communities, the isolation of PGPB suggests that the rhizosphere bacteria are driven by the plant species, soil type, and fire regime, and are potentially associated with a wide range of agricultural, environmental, and industrial applications. Thus, the rhizosphere of African savannah ecosystems seems to be an untapped source of bacterial species and strains that should be further exploited for bio-based solutions.
AB - (1) Aims: Assessing bacterial diversity and plant-growth-promoting functions in the rhizosphere of the native African trees Colophospermum mopane and Combretum apiculatum in three landscapes of the Limpopo National Park (Mozambique), subjected to two fire regimes. (2) Methods: Bacterial communities were identified through Illumina Miseq sequencing of the 16S rRNA gene amplicons, followed by culture dependent methods to isolate plant growth-promoting bacteria (PGPB). Plant growth-promoting traits of the cultivable bacterial fraction were further analyzed. To screen for the presence of nitrogen-fixing bacteria, the promiscuous tropical legume Vigna unguiculata was used as a trap host. The taxonomy of all purified isolates was genetically verified by 16S rRNA gene Sanger sequencing. (3) Results: Bacterial community results indicated that fire did not drive major changes in bacterial abundance. However, culture-dependent methods allowed the differentiation of bacterial communities between the sampled sites, which were particularly enriched in Proteobacteria with a wide range of plant-beneficial traits, such as plant protection, plant nutrition, and plant growth. Bradyrhizobium was the most frequent symbiotic bacteria trapped in cowpea nodules coexisting with other endophytic bacteria. (4) Conclusion: Although the global analysis did not show significant differences between landscapes or sites with different fire regimes, probably due to the fast recovery of bacterial communities, the isolation of PGPB suggests that the rhizosphere bacteria are driven by the plant species, soil type, and fire regime, and are potentially associated with a wide range of agricultural, environmental, and industrial applications. Thus, the rhizosphere of African savannah ecosystems seems to be an untapped source of bacterial species and strains that should be further exploited for bio-based solutions.
KW - 16SrRNA
KW - Combretum
KW - Fire
KW - Limpopo National Park
KW - Mopane
KW - Plant growth-promoting bacteria
KW - Rhizosphere
UR - http://www.scopus.com/inward/record.url?scp=85090610692&partnerID=8YFLogxK
U2 - 10.3390/microorganisms8091291
DO - 10.3390/microorganisms8091291
M3 - Article
AN - SCOPUS:85090610692
SN - 2076-2607
VL - 8
SP - 1
EP - 21
JO - Microorganisms
JF - Microorganisms
IS - 9
M1 - 1291
ER -