Abstract
Fluorescence in situ hybridization (FISH) has been widely used for the detection and identification of microorganisms in their natural environments. In this chapter we describe the use of a simple FISH-based protocol to detect and identify clinically relevant yeast species in culture and biological samples using Cryptococcus neoformans as a model. After fixation of cells with paraformaldehyde, the same are embedded in hybridization buffer containing specific fluorochrome-labeled oligonucleotide probes. After incubation and a subsequent washing step for removing unbound probes, samples are analyzed by epifluorescence microscopy.
| Original language | English |
|---|---|
| Title of host publication | Fungal Diagnostics - Methods in Molecular Biology |
| Editors | L O'Connor, B Glynn |
| Place of Publication | Totowa, USA |
| Publisher | Humana Press |
| Pages | 71-82 |
| Volume | 968 |
| ISBN (Print) | 978-1-62703-256-8 |
| Publication status | Published - 1 Jan 2013 |
