Microscopic detection of yeasts using fluorescence in situ hybridization.

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

3 Citations (Scopus)


Fluorescence in situ hybridization (FISH) has been widely used for the detection and identification of microorganisms in their natural environments. In this chapter we describe the use of a simple FISH-based protocol to detect and identify clinically relevant yeast species in culture and biological samples using Cryptococcus neoformans as a model. After fixation of cells with paraformaldehyde, the same are embedded in hybridization buffer containing specific fluorochrome-labeled oligonucleotide probes. After incubation and a subsequent washing step for removing unbound probes, samples are analyzed by epifluorescence microscopy.
Original languageEnglish
Title of host publicationFungal Diagnostics - Methods in Molecular Biology
EditorsL O'Connor, B Glynn
Place of PublicationTotowa, USA
PublisherHumana Press
ISBN (Print)978-1-62703-256-8
Publication statusPublished - 1 Jan 2013


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