Microscopic detection of yeasts using fluorescence in situ hybridization.

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

Fluorescence in situ hybridization (FISH) has been widely used for the detection and identification of microorganisms in their natural environments. In this chapter we describe the use of a simple FISH-based protocol to detect and identify clinically relevant yeast species in culture and biological samples using Cryptococcus neoformans as a model. After fixation of cells with paraformaldehyde, the same are embedded in hybridization buffer containing specific fluorochrome-labeled oligonucleotide probes. After incubation and a subsequent washing step for removing unbound probes, samples are analyzed by epifluorescence microscopy.
Original languageEnglish
Title of host publicationFungal Diagnostics - Methods in Molecular Biology
EditorsL O'Connor, B Glynn
Place of PublicationTotowa, USA
PublisherHumana Press
Pages71-82
Volume968
ISBN (Print)978-1-62703-256-8
Publication statusPublished - 1 Jan 2013

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