MicroRNA profiling in plasma or serum using quantitative RT-PCR.

Ana Lúcia Monteiro Durão Leitão

doi:10.1007/978-1-4939-1062-5_11

MicroRNA profiling in plasma or serum using quantitative RT-PCR.

Ana Lúcia Monteiro Durão Leitão

Research output: Chapter in Book/Report/Conference proceeding › Chapter

17 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) are important cellular modulators that regulate gene expression at the posttranscriptional level. Circulating miRNAs present in human plasma or serum have recently become an emerging field in biomedical research, mostly due to its potential applications in the diagnosis and prognosis of several diseases. Although miRNA profiling in biofluids holds great promise, there are several challenges to overcome. Here, we present an experimental procedure for profiling miRNA in plasma or serum with high sensitivity and specificity using qRT-PCR. This method is also suitable for studying miRNAs in other body fluids or clinical samples that also contain low amounts of RNA.

Original language	Unknown
Title of host publication	RNA mapping: methods and protocols
Editors	M.L. Alvarez, M. Nourbakhsh
Place of Publication	New York, USA
Publisher	Humana Press
Pages	121-9
Volume	1182
ISBN (Print)	978-1-4939-1061-8
DOIs	https://doi.org/10.1007/978-1-4939-1062-5_11
Publication status	Published - 1 Jan 2014

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10.1007/978-1-4939-1062-5_11

Cite this

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abstract = "MicroRNAs (miRNAs) are important cellular modulators that regulate gene expression at the posttranscriptional level. Circulating miRNAs present in human plasma or serum have recently become an emerging field in biomedical research, mostly due to its potential applications in the diagnosis and prognosis of several diseases. Although miRNA profiling in biofluids holds great promise, there are several challenges to overcome. Here, we present an experimental procedure for profiling miRNA in plasma or serum with high sensitivity and specificity using qRT-PCR. This method is also suitable for studying miRNAs in other body fluids or clinical samples that also contain low amounts of RNA.",

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AB - MicroRNAs (miRNAs) are important cellular modulators that regulate gene expression at the posttranscriptional level. Circulating miRNAs present in human plasma or serum have recently become an emerging field in biomedical research, mostly due to its potential applications in the diagnosis and prognosis of several diseases. Although miRNA profiling in biofluids holds great promise, there are several challenges to overcome. Here, we present an experimental procedure for profiling miRNA in plasma or serum with high sensitivity and specificity using qRT-PCR. This method is also suitable for studying miRNAs in other body fluids or clinical samples that also contain low amounts of RNA.

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BT - RNA mapping: methods and protocols

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A2 - Nourbakhsh, M.

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