MicroRNA profiling in plasma or serum using quantitative RT-PCR.

Research output: Chapter in Book/Report/Conference proceedingChapter

12 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) are important cellular modulators that regulate gene expression at the posttranscriptional level. Circulating miRNAs present in human plasma or serum have recently become an emerging field in biomedical research, mostly due to its potential applications in the diagnosis and prognosis of several diseases. Although miRNA profiling in biofluids holds great promise, there are several challenges to overcome. Here, we present an experimental procedure for profiling miRNA in plasma or serum with high sensitivity and specificity using qRT-PCR. This method is also suitable for studying miRNAs in other body fluids or clinical samples that also contain low amounts of RNA.
Original languageUnknown
Title of host publicationRNA mapping: methods and protocols
EditorsM.L. Alvarez, M. Nourbakhsh
Place of PublicationNew York, USA
PublisherHumana Press, Springer
Pages121-9
Volume1182
ISBN (Print)978-1-4939-1061-8
DOIs
Publication statusPublished - 1 Jan 2014

Cite this