TY - JOUR
T1 - Microneedle Arrays of Polyhydroxyalkanoate by Laser-Based Micromolding Technique
AU - Silvestre, Sara L.
AU - Araújo, Diana
AU - Marques, Ana C.
AU - Pires, Carolina
AU - Matos, Mariana
AU - Alves, Vítor
AU - Martins, Rodrigo
AU - Freitas, Filomena
AU - Reis, Maria A. M.
AU - Fortunato, Elvira
N1 - UID/CTM/50025/2019;
UID/Muld/04378/2013;
PTDC/BBB-BIO/1889/2014;
Ph.D. grants SFRH/BD/115173/2016; BD/140829/2018.
This work is part of the Master Thesis in Micro and Nano technologies Engineering defended by Sara Silvestre at FCT NOVA in 2018 titled "Biopolymer based microneedles patch by laser technology for biomedical applications" and the Master Thesis in Materials Engineering defended by Ana C.P. at FCT NOVA titled "Laser technology and 3D printing for production of biopolymer-based hollow microneedles patch for biomedical applications".
PY - 2020/9/21
Y1 - 2020/9/21
N2 - Although many delivery systems have been proposed to improve drug permeation through the skin, all of them suffer from several limitations. This drives a continuous search for innovative systems that can provide safe and effective transdermal drug delivery solutions. In line with this, microneedle (MN) arrays, a hybrid combination of hypodermic injections and transdermal patches, have been proposed. MNs consist of microscale needles that can pierce the skin by a simple, minimally invasive, and painless route, enabling the transport of drugs and macromolecules into the human body. This study reports, for the first time, the use of a biobased, biodegradable, and biocompatible polymer, poly(3-hydroxybutyrate-co-3-hydroxyvalerate), P(3HB-co-3HV), for the fabrication of a biopolymer-based MN patch. Molds of poly(dimethylsiloxane) were prepared by direct laser writing technology, a low-cost and mask-less technology, and used to produce biodegradable P(3HB-co-3HV) MNs by a thermosetting process. The best results were obtained with a laser power of 30 W at 0.15 m/s with a spiral model as the pattern. The obtained MNs had a length of 0.69 mm and a diameter of 0.33 mm, ideal for painless penetration of the skin. Additionally, the produced MNs had good mechanical properties and the ability to be successfully impregnated with the fluorescent dye Rhodamine 6G. These features render P(3HB-co-3HV) a promising material for the development of MNs with improved functionality.
AB - Although many delivery systems have been proposed to improve drug permeation through the skin, all of them suffer from several limitations. This drives a continuous search for innovative systems that can provide safe and effective transdermal drug delivery solutions. In line with this, microneedle (MN) arrays, a hybrid combination of hypodermic injections and transdermal patches, have been proposed. MNs consist of microscale needles that can pierce the skin by a simple, minimally invasive, and painless route, enabling the transport of drugs and macromolecules into the human body. This study reports, for the first time, the use of a biobased, biodegradable, and biocompatible polymer, poly(3-hydroxybutyrate-co-3-hydroxyvalerate), P(3HB-co-3HV), for the fabrication of a biopolymer-based MN patch. Molds of poly(dimethylsiloxane) were prepared by direct laser writing technology, a low-cost and mask-less technology, and used to produce biodegradable P(3HB-co-3HV) MNs by a thermosetting process. The best results were obtained with a laser power of 30 W at 0.15 m/s with a spiral model as the pattern. The obtained MNs had a length of 0.69 mm and a diameter of 0.33 mm, ideal for painless penetration of the skin. Additionally, the produced MNs had good mechanical properties and the ability to be successfully impregnated with the fluorescent dye Rhodamine 6G. These features render P(3HB-co-3HV) a promising material for the development of MNs with improved functionality.
KW - biopolymer
KW - direct laser writing
KW - drug delivery
KW - microneedles arrays
KW - poly(3-hydroxybutyrate- co-3-hydroxyvalerate)
UR - http://www.scopus.com/inward/record.url?scp=85092788942&partnerID=8YFLogxK
U2 - 10.1021/acsabm.0c00570
DO - 10.1021/acsabm.0c00570
M3 - Article
AN - SCOPUS:85092788942
SN - 2576-6422
VL - 3
SP - 5856
EP - 5864
JO - ACS Applied Bio Materials
JF - ACS Applied Bio Materials
IS - 9
ER -