Microfluidics-driven fabrication of a low cost and ultrasensitive SERS-based paper biosensor

Alexandra Teixeira, Juan F. Hernández-Rodríguez, Lei Wu, Kevin Oliveira, Krishna Kant, Paulina Piairo, Lorena Diéguez, Sara Abalde-Cela

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)
6 Downloads (Pure)


Surface-enhanced Raman scattering (SERS) spectroscopy stands out due to its sensitivity, selectivity, and multiplex ability. The development of ready-to-use, simple, and low-cost SERS substrates is one of the main challenges of the field. In this paper, the intrinsic reproducibility of microfluidics technology was used for the fabrication of self-assembled nanoparticle structures over a paper film. The paper SERS substrates were fabricated by assembling anisotropic particles, gold nanostars (GNSs), and nanorods (NRs) onto paper to offer an extra enhancement to reach ultra-sensitive detection limits. A polydimethylsiloxane PDMS-paper hybrid device was used to control the drying kinetics of the nanoparticles over the paper substrate. This method allowed a high reproducibility and homogeneity of the fabrication of SERS substrates that reach limits of detection down to the picomolar range. This simple and low-cost fabrication of a paper-based sensing device was tested for the discrimination of different cell lineages.

Original languageEnglish
Article number1387
JournalApplied Sciences (Switzerland)
Issue number7
Publication statusPublished - 1 Apr 2019


  • Hybrid
  • Microfluidics
  • Nano-biosensors
  • Nanoparticles
  • Nanotechnology
  • Self-assembly
  • SERS


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