TY - JOUR
T1 - Microbial production of medium-chain length polyhydroxyalkanoates
AU - Silva, Juliana B.
AU - Pereira, João R.
AU - Marreiros, Bruno C.
AU - Reis, Maria A. M.
AU - Freitas, Filomena
N1 - info:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F147518%2F2019/PT#
This work was supported by FCT/MCTES through the project Mar-Noruega/0001/2016 and the Research Unit on Applied Molecular Biosciences (UCIBIO) [grant number UID/04378/2020]; ; Bruno C. Marreiros acknowledges the financial support from FCT/MCTES through the project PTDC/BTA-BTA/30902/2017.
PY - 2021/3
Y1 - 2021/3
N2 - Medium-chain-length poly(hydroxyalkanoates), mcl-PHAs, are naturally produced polymers characterized by material properties that include low crystallinity and low melting and glass transition temperatures, which render them suitable for diverse applications. These biodegradable and biocompatible biopolymers are accumulated as intracellular energy and carbon reserves by a wide range of bacteria, mainly, of the genus Pseudomonas. Despite their valuable properties, mcl-PHAs commercial development is still limited, mostly due to the high costs associated with their production and extraction. To overcome such problems, several approaches are being pursued comprising: the search for higher yielding strains, both wild-type and engineered bacteria; the utilization of low-cost feedstocks, such as wastes; and the development of environmentally and economically sustainable downstream procedures for polymer recovery and purification. Intensive research is also being done for the development of mcl-PHA-based materials for use in different areas, especially in high-value applications, such as biomedicine and drug delivery, in which the product's performance may justify its higher cost.
AB - Medium-chain-length poly(hydroxyalkanoates), mcl-PHAs, are naturally produced polymers characterized by material properties that include low crystallinity and low melting and glass transition temperatures, which render them suitable for diverse applications. These biodegradable and biocompatible biopolymers are accumulated as intracellular energy and carbon reserves by a wide range of bacteria, mainly, of the genus Pseudomonas. Despite their valuable properties, mcl-PHAs commercial development is still limited, mostly due to the high costs associated with their production and extraction. To overcome such problems, several approaches are being pursued comprising: the search for higher yielding strains, both wild-type and engineered bacteria; the utilization of low-cost feedstocks, such as wastes; and the development of environmentally and economically sustainable downstream procedures for polymer recovery and purification. Intensive research is also being done for the development of mcl-PHA-based materials for use in different areas, especially in high-value applications, such as biomedicine and drug delivery, in which the product's performance may justify its higher cost.
KW - Bacterial cultivation
KW - Extraction
KW - Medium-chain length poly(hydroxyalkanoates) (mcl-PHAs)
KW - Metabolic pathways
UR - http://www.scopus.com/inward/record.url?scp=85100680863&partnerID=8YFLogxK
U2 - 10.1016/j.procbio.2021.01.020
DO - 10.1016/j.procbio.2021.01.020
M3 - Review article
AN - SCOPUS:85100680863
SN - 1359-5113
VL - 102
SP - 393
EP - 407
JO - Process Biochemistry
JF - Process Biochemistry
ER -