Microbial community analysis with a high PHA storage capacity

L. S. Serafim; P. C. Lemos; S. Rossetti; C. Levantesi; V. Tandoi; M. A. M. Reis

doi:10.2166/wst.2006.386

Microbial community analysis with a high PHA storage capacity

L. S. Serafim, P. C. Lemos, S. Rossetti, C. Levantesi, V. Tandoi, M. A. M. Reis

Research output: Contribution to journal › Conference article › peer-review

36 Citations (Scopus)

Abstract

Activated sludge was submitted to aerobic dynamic substrate feeding for the production of biodegradable plastics. Two sequencing batch reactors were operated with acetate or propionate as sole carbon substrates. With acetate a homopolymer of polyhydroxybutyrate (PHB) was obtained and with propionate a copolymer of hydroxybutyrate and hydroxyvalerate P(HB/HV) was produced. Three main morphotypes were identified in both sludges: two belong to the Alphaproteobacteria class and the third to the Betaproteobacteria class. Bacilli belonging to Betaproteobacteria were shown by FISH analysis, applied in combination with Nile Blue post-staining, to be the main responsible for PHAs storage. The latter were affiliated to Azoarcus genus within Betaproteobacteria.

Original language	English
Pages (from-to)	183-188
Number of pages	6
Journal	Water Science and Technology
Volume	54
Issue number	1
DOIs	https://doi.org/10.2166/wst.2006.386
Publication status	Published - 7 Aug 2006
Event	4th IWA Activated Sludge Population Dynamics Conference (ASPD4) - Gold Coast, Australia Duration: 17 Jul 2005 → 20 Jul 2005

Keywords

Acetate
Aerobic dynamic substrate feeding
FISH
Polyhydroxyalkanoates
Propionate
SBR

Access to Document

10.2166/wst.2006.386Licence: CC BY-NC

Cite this

@article{b25c35a47d0d4923a2d3a7e7c4e20138,

title = "Microbial community analysis with a high PHA storage capacity",

abstract = "Activated sludge was submitted to aerobic dynamic substrate feeding for the production of biodegradable plastics. Two sequencing batch reactors were operated with acetate or propionate as sole carbon substrates. With acetate a homopolymer of polyhydroxybutyrate (PHB) was obtained and with propionate a copolymer of hydroxybutyrate and hydroxyvalerate P(HB/HV) was produced. Three main morphotypes were identified in both sludges: two belong to the Alphaproteobacteria class and the third to the Betaproteobacteria class. Bacilli belonging to Betaproteobacteria were shown by FISH analysis, applied in combination with Nile Blue post-staining, to be the main responsible for PHAs storage. The latter were affiliated to Azoarcus genus within Betaproteobacteria.",

keywords = "Acetate, Aerobic dynamic substrate feeding, FISH, Polyhydroxyalkanoates, Propionate, SBR",

author = "Serafim, {L. S.} and Lemos, {P. C.} and S. Rossetti and C. Levantesi and V. Tandoi and Reis, {M. A. M.}",

year = "2006",

month = aug,

day = "7",

doi = "10.2166/wst.2006.386",

language = "English",

volume = "54",

pages = "183--188",

journal = "Water Science and Technology",

issn = "0273-1223",

publisher = "IWA Publishing",

number = "1",

note = "4th IWA Activated Sludge Population Dynamics Conference (ASPD4) ; Conference date: 17-07-2005 Through 20-07-2005",

}

TY - JOUR

T1 - Microbial community analysis with a high PHA storage capacity

AU - Serafim, L. S.

AU - Lemos, P. C.

AU - Rossetti, S.

AU - Levantesi, C.

AU - Tandoi, V.

AU - Reis, M. A. M.

PY - 2006/8/7

Y1 - 2006/8/7

N2 - Activated sludge was submitted to aerobic dynamic substrate feeding for the production of biodegradable plastics. Two sequencing batch reactors were operated with acetate or propionate as sole carbon substrates. With acetate a homopolymer of polyhydroxybutyrate (PHB) was obtained and with propionate a copolymer of hydroxybutyrate and hydroxyvalerate P(HB/HV) was produced. Three main morphotypes were identified in both sludges: two belong to the Alphaproteobacteria class and the third to the Betaproteobacteria class. Bacilli belonging to Betaproteobacteria were shown by FISH analysis, applied in combination with Nile Blue post-staining, to be the main responsible for PHAs storage. The latter were affiliated to Azoarcus genus within Betaproteobacteria.

AB - Activated sludge was submitted to aerobic dynamic substrate feeding for the production of biodegradable plastics. Two sequencing batch reactors were operated with acetate or propionate as sole carbon substrates. With acetate a homopolymer of polyhydroxybutyrate (PHB) was obtained and with propionate a copolymer of hydroxybutyrate and hydroxyvalerate P(HB/HV) was produced. Three main morphotypes were identified in both sludges: two belong to the Alphaproteobacteria class and the third to the Betaproteobacteria class. Bacilli belonging to Betaproteobacteria were shown by FISH analysis, applied in combination with Nile Blue post-staining, to be the main responsible for PHAs storage. The latter were affiliated to Azoarcus genus within Betaproteobacteria.

KW - Acetate

KW - Aerobic dynamic substrate feeding

KW - FISH

KW - Polyhydroxyalkanoates

KW - Propionate

KW - SBR

UR - http://www.scopus.com/inward/record.url?scp=33746637126&partnerID=8YFLogxK

U2 - 10.2166/wst.2006.386

DO - 10.2166/wst.2006.386

M3 - Conference article

C2 - 16898151

AN - SCOPUS:33746637126

SN - 0273-1223

VL - 54

SP - 183

EP - 188

JO - Water Science and Technology

JF - Water Science and Technology

IS - 1

T2 - 4th IWA Activated Sludge Population Dynamics Conference (ASPD4)

Y2 - 17 July 2005 through 20 July 2005

ER -

Microbial community analysis with a high PHA storage capacity

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this