Microarray analyses reveal that plant mutagenesis may induce more transcriptomic changes than transgene insertion

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

Controversy regarding genetically modified (GM) plants and their potential impact on human health contrasts with the tacit acceptance of other plants that were also modified, but not considered as GM products (e.g., varieties raised through conventional breeding such as mutagenesis). What is beyond the phenotype of these improved plants? Should mutagenized plants be treated differently from transgenics? We have evaluated the extent of transcriptome modification occurring during rice improvement through transgenesis versus mutation breeding. We used oligonucleotide microarrays to analyze gene expression in four different pools of four types of rice plants and respective controls: (i) a γ-irradiated stable mutant, (ii) the M1 generation of a 100-Gy γ-irradiated plant, (iii) a stable transgenic plant obtained for production of an anticancer antibody, and (iv) the T1 generation of a transgenic plant produced aiming for abiotic stress improvement, and all of the unmodified original genotypes as controls. We found that the improvement of a plant variety through the acquisition of a new desired trait, using either mutagenesis or transgenesis, may cause stress and thus lead to an altered expression of untargeted genes. In all of the cases studied, the observed alteration was more extensive in mutagenized than in transgenic plants. We propose that the safety assessment of improved plant varieties should be carried out on a case-by-case basis and not simply restricted to foods obtained through genetic engineering.

Original languageEnglish
Pages (from-to)3640-3645
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number9
DOIs
Publication statusPublished - 4 Mar 2008

Keywords

  • γ-irradiation
  • Food safety evaluation
  • Genetic engineering
  • Genetically modified organisms
  • Rice

Fingerprint Dive into the research topics of 'Microarray analyses reveal that plant mutagenesis may induce more transcriptomic changes than transgene insertion'. Together they form a unique fingerprint.

  • Cite this