Abstract
The main objective of this work was to evaluate arsenic effects on metallothionein (MT) induction by exposing a freshwater Asiatic clam (Corbicula fluminea) to different concentrations of this metalloid. The presence of MT-like proteins was detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis and compared with a standard rabbit MT. In addition, the polarographic response showed good correspondence between standard MT and MT-like curves from C. fluminea, allowing MT quantification. The results show that clams exposed to different concentrations of arsenic are able to induce significant levels of MTs. Although variability was found in MT induction, significant differences in MT levels were found after 28 days of exposure in all treatments in comparison with the controls, suggesting that exposure to arsenic induced MT-like proteins in C. fluminea.
| Original language | English |
|---|---|
| Pages (from-to) | 589-598 |
| Number of pages | 10 |
| Journal | Biomarkers |
| Volume | 12 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Nov 2007 |
Keywords
- Arsenic
- Biosynthesis
- Corbicula fluminea
- Metallothionein
- arsenic
- fresh water
- metallothionein
- animal experiment
- animal tissue
- arsenic poisoning
- article
- clam
- controlled study
- nonhuman
- polarography
- polyacrylamide gel electrophoresis
- protein analysis
- protein blood level
- protein induction
- protein synthesis
- quantitative analysis
- Animals
- Arsenicals
- Biological Markers
- Corbicula
- Dose-Response Relationship, Drug
- Electrophoresis, Polyacrylamide Gel
- Environmental Monitoring
- Fresh Water
- Polarography
- Time Factors
- Up-Regulation
- Water Pollutants, Chemical
- Bivalvia
- Oryctolagus cuniculus