Metal centers in biomolecular solid-state NMR

José Malanho Silva; Linda Cerofolini; Stefano Giuntini; Vito Calderone; Carlos F. G. C. Geraldes; Anjos L. Macedo; Giacomo Parigi; Marco Fragai; Enrico Ravera; Claudio Luchinat

doi:10.1016/j.jsb.2018.11.013

Metal centers in biomolecular solid-state NMR

José Malanho Silva, Linda Cerofolini, Stefano Giuntini, Vito Calderone, Carlos F. G. C. Geraldes, Anjos L. Macedo, Giacomo Parigi, Marco Fragai, Enrico Ravera, Claudio Luchinat

UCIBIO - Applied Molecular Biosciences Unit

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Solid state NMR (SSNMR) has earned a substantial success in the characterization of paramagnetic systems over the last decades. Nowadays, the resolution and sensitivity of solid state NMR in biological molecules has improved significantly and these advancements can be translated into the study of paramagnetic biomolecules. However, the electronic properties of different metal centers affect the quality of their SSNMR spectra differently, and not all systems turn out to be equally easy to approach by this technique. In this review we will try to give an overview of the properties of different paramagnetic centers and how they can be used to increase the chances of experimental success.

Original language	English
Pages (from-to)	99-109
Journal	Journal Of Structural Biology
Volume	206
Issue number	1
DOIs	https://doi.org/10.1016/j.jsb.2018.11.013
Publication status	Published - 1 Apr 2019

Keywords

Biosolids
Electronic structure
Paramagnetic NMR
Resolution
Sensitivity

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10.1016/j.jsb.2018.11.013Licence: CC BY-NC-ND

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title = "Metal centers in biomolecular solid-state NMR",

abstract = "Solid state NMR (SSNMR) has earned a substantial success in the characterization of paramagnetic systems over the last decades. Nowadays, the resolution and sensitivity of solid state NMR in biological molecules has improved significantly and these advancements can be translated into the study of paramagnetic biomolecules. However, the electronic properties of different metal centers affect the quality of their SSNMR spectra differently, and not all systems turn out to be equally easy to approach by this technique. In this review we will try to give an overview of the properties of different paramagnetic centers and how they can be used to increase the chances of experimental success.",

keywords = "Biosolids, Electronic structure, Paramagnetic NMR, Resolution, Sensitivity",

author = "{Malanho Silva}, Jos{\'e} and Linda Cerofolini and Stefano Giuntini and Vito Calderone and Geraldes, {Carlos F. G. C.} and Macedo, {Anjos L.} and Giacomo Parigi and Marco Fragai and Enrico Ravera and Claudio Luchinat",

note = "This work has been supported by Fondazione Cassa di Risparmio di Firenze , the European Commission (contract #675858). The authors acknowledge the use of resources of Instruct-ERIC, an ESFRI Landmark, supported by national member subscriptions. J.M.S. acknowledges FCT for the doctoral fellowship PD/BD/135180/2017 integrated in the PhD Program in NMR applied to chemistry, materials and biosciences.",

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doi = "10.1016/j.jsb.2018.11.013",

language = "English",

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AU - Malanho Silva, José

AU - Cerofolini, Linda

AU - Giuntini, Stefano

AU - Calderone, Vito

AU - Geraldes, Carlos F. G. C.

AU - Macedo, Anjos L.

AU - Parigi, Giacomo

AU - Fragai, Marco

AU - Ravera, Enrico

AU - Luchinat, Claudio

N1 - This work has been supported by Fondazione Cassa di Risparmio di Firenze , the European Commission (contract #675858). The authors acknowledge the use of resources of Instruct-ERIC, an ESFRI Landmark, supported by national member subscriptions. J.M.S. acknowledges FCT for the doctoral fellowship PD/BD/135180/2017 integrated in the PhD Program in NMR applied to chemistry, materials and biosciences.

PY - 2019/4/1

Y1 - 2019/4/1

N2 - Solid state NMR (SSNMR) has earned a substantial success in the characterization of paramagnetic systems over the last decades. Nowadays, the resolution and sensitivity of solid state NMR in biological molecules has improved significantly and these advancements can be translated into the study of paramagnetic biomolecules. However, the electronic properties of different metal centers affect the quality of their SSNMR spectra differently, and not all systems turn out to be equally easy to approach by this technique. In this review we will try to give an overview of the properties of different paramagnetic centers and how they can be used to increase the chances of experimental success.

AB - Solid state NMR (SSNMR) has earned a substantial success in the characterization of paramagnetic systems over the last decades. Nowadays, the resolution and sensitivity of solid state NMR in biological molecules has improved significantly and these advancements can be translated into the study of paramagnetic biomolecules. However, the electronic properties of different metal centers affect the quality of their SSNMR spectra differently, and not all systems turn out to be equally easy to approach by this technique. In this review we will try to give an overview of the properties of different paramagnetic centers and how they can be used to increase the chances of experimental success.

KW - Biosolids

KW - Electronic structure

KW - Paramagnetic NMR

KW - Resolution

KW - Sensitivity

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JO - Journal Of Structural Biology

JF - Journal Of Structural Biology

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Metal centers in biomolecular solid-state NMR

Abstract

Keywords

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