Metabolic Profiling of Insect Cell Lines: UnveilingCell Line Determinants Behind System’s Productivity

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Abstract

Baculovirus infection boosts the host biosyntheticactivity towards the production of viral componentsand the recombinant protein of interest, hyper-productivephenotypes being the result of a successful adaptation of thecellular network to that scenario. Spodoptera frugiperda derivedSf9 and Trichoplusia ni derived High Five cell lines have a majortrack record for the production of recombinant proteins, withHigh Five cells presenting higher productivities. A metabolicprofiling of the two insect cell lines was pursued to underpinspecific cellular traits behind productive phenotypes. Multivariateanalysis identified cell-line dependent metabolicsignatures linked to productivity. Pathway analysis highlightedcellular pathways of paramount importance in supportinginfection and protein production. Moreover, better producerphenotypes proved to be correlated with the capacity of cellsto shift their metabolism in favor of energy-generatingpathways to fuel biosynthesis, a scenario observed in the HighFive cell line. Metabolomic profiling allowed us to identifymetabolic pathways involved in infection and recombinantprotein production, which can be selected as targets forfurther improvement of the system.
Original languageUnknown
Pages (from-to)816–828
JournalBiotechnology and Bioengineering
Volume111
Issue number4
DOIs
Publication statusPublished - 1 Jan 2014

Cite this

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title = "Metabolic Profiling of Insect Cell Lines: UnveilingCell Line Determinants Behind System’s Productivity",
abstract = "Baculovirus infection boosts the host biosyntheticactivity towards the production of viral componentsand the recombinant protein of interest, hyper-productivephenotypes being the result of a successful adaptation of thecellular network to that scenario. Spodoptera frugiperda derivedSf9 and Trichoplusia ni derived High Five cell lines have a majortrack record for the production of recombinant proteins, withHigh Five cells presenting higher productivities. A metabolicprofiling of the two insect cell lines was pursued to underpinspecific cellular traits behind productive phenotypes. Multivariateanalysis identified cell-line dependent metabolicsignatures linked to productivity. Pathway analysis highlightedcellular pathways of paramount importance in supportinginfection and protein production. Moreover, better producerphenotypes proved to be correlated with the capacity of cellsto shift their metabolism in favor of energy-generatingpathways to fuel biosynthesis, a scenario observed in the HighFive cell line. Metabolomic profiling allowed us to identifymetabolic pathways involved in infection and recombinantprotein production, which can be selected as targets forfurther improvement of the system.",
keywords = "recombinant protein production, metabolic pathwayanalysis, metabolomics, virus–host interactions, Baculovirus expression vectorsystem",
author = "Carrondo, {Manuel Jos{\'e} Teixeira} and Alves, {Paula Maria}",
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T1 - Metabolic Profiling of Insect Cell Lines: UnveilingCell Line Determinants Behind System’s Productivity

AU - Carrondo, Manuel José Teixeira

AU - Alves, Paula Maria

N1 - Sem PDF conforme despacho.

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Baculovirus infection boosts the host biosyntheticactivity towards the production of viral componentsand the recombinant protein of interest, hyper-productivephenotypes being the result of a successful adaptation of thecellular network to that scenario. Spodoptera frugiperda derivedSf9 and Trichoplusia ni derived High Five cell lines have a majortrack record for the production of recombinant proteins, withHigh Five cells presenting higher productivities. A metabolicprofiling of the two insect cell lines was pursued to underpinspecific cellular traits behind productive phenotypes. Multivariateanalysis identified cell-line dependent metabolicsignatures linked to productivity. Pathway analysis highlightedcellular pathways of paramount importance in supportinginfection and protein production. Moreover, better producerphenotypes proved to be correlated with the capacity of cellsto shift their metabolism in favor of energy-generatingpathways to fuel biosynthesis, a scenario observed in the HighFive cell line. Metabolomic profiling allowed us to identifymetabolic pathways involved in infection and recombinantprotein production, which can be selected as targets forfurther improvement of the system.

AB - Baculovirus infection boosts the host biosyntheticactivity towards the production of viral componentsand the recombinant protein of interest, hyper-productivephenotypes being the result of a successful adaptation of thecellular network to that scenario. Spodoptera frugiperda derivedSf9 and Trichoplusia ni derived High Five cell lines have a majortrack record for the production of recombinant proteins, withHigh Five cells presenting higher productivities. A metabolicprofiling of the two insect cell lines was pursued to underpinspecific cellular traits behind productive phenotypes. Multivariateanalysis identified cell-line dependent metabolicsignatures linked to productivity. Pathway analysis highlightedcellular pathways of paramount importance in supportinginfection and protein production. Moreover, better producerphenotypes proved to be correlated with the capacity of cellsto shift their metabolism in favor of energy-generatingpathways to fuel biosynthesis, a scenario observed in the HighFive cell line. Metabolomic profiling allowed us to identifymetabolic pathways involved in infection and recombinantprotein production, which can be selected as targets forfurther improvement of the system.

KW - recombinant protein production

KW - metabolic pathwayanalysis

KW - metabolomics

KW - virus–host interactions

KW - Baculovirus expression vectorsystem

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SP - 816

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JO - Biotechnology and Bioengineering

JF - Biotechnology and Bioengineering

SN - 0006-3592

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