TY - JOUR
T1 - Metabolic modeling of the substrate competition among multiple VFAs for PHA production by mixed microbial cultures
AU - Wang, Xiaofei
AU - Carvalho, Gilda
AU - Reis, Maria A. M.
AU - Oehmen, Adrian
N1 - Sem PDF conforme despacho.
The authors acknowledge financial support from MERMAID project "Microbial Resource Management and Engineering for Closing the Urban Water Cycle", a Marie Sklodowska-Curie Initial Training Network, under grant number 607492. This work was also supported by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728).
PY - 2018/8/20
Y1 - 2018/8/20
N2 - Mixed microbial cultures (MMCs) are an effective method for polyhydroxyalkanoates (PHA) production. There are several models established to describe the metabolism of this process. Substrate competition was commonly found in the uptake of multiple volatile fatty acids (VFAs), thus this behavior should be considered in process modeling. In this study, a metabolic model is developed to describe for the first time the substrate competition among four commonly used VFAs (acetate, propionate, butyrate and valerate) in PHA production by MMCs. An inhibition parameter was introduced to describe the inhibition of butyrate and valerate on the uptake of acetate and propionate. The modeled results showed good agreement with experimental data within 95% confidence interval. This model enhanced the understanding of the metabolism of substrate uptake in PHA production, providing a successful approach to predict PHA composition and optimize the process efficiency when four competing VFAs are supplied.
AB - Mixed microbial cultures (MMCs) are an effective method for polyhydroxyalkanoates (PHA) production. There are several models established to describe the metabolism of this process. Substrate competition was commonly found in the uptake of multiple volatile fatty acids (VFAs), thus this behavior should be considered in process modeling. In this study, a metabolic model is developed to describe for the first time the substrate competition among four commonly used VFAs (acetate, propionate, butyrate and valerate) in PHA production by MMCs. An inhibition parameter was introduced to describe the inhibition of butyrate and valerate on the uptake of acetate and propionate. The modeled results showed good agreement with experimental data within 95% confidence interval. This model enhanced the understanding of the metabolism of substrate uptake in PHA production, providing a successful approach to predict PHA composition and optimize the process efficiency when four competing VFAs are supplied.
KW - Inhibition
KW - Metabolic modeling
KW - Polyhydroxyalkanoates (PHAs)
KW - Substrate competition
UR - http://www.scopus.com/inward/record.url?scp=85048747528&partnerID=8YFLogxK
U2 - 10.1016/j.jbiotec.2018.06.342
DO - 10.1016/j.jbiotec.2018.06.342
M3 - Article
C2 - 29932948
AN - SCOPUS:85048747528
VL - 280
SP - 62
EP - 69
JO - Journal of Biotechnology
JF - Journal of Biotechnology
SN - 0168-1656
ER -