@article{7986d92a4dbb450e8eaff6920e8becea,
title = "Measurement of adsorption constants of laccase on gold nanoparticles to evaluate the enhancement in enzyme activity of adsorbed laccase",
abstract = "Adsorption of enzymes to nanoparticles and the mechanisms responsible for enzyme activity modulation of adsorbed enzymes are not well understood. In this work, gold nanoparticles were used for electrostatic adsorption of a plant-derived laccase. Adsorption constants were determined by four independent techniques: dynamic light scattering, electrophoretic light scattering, agarose gel electrophoresis and fluorescence quenching. Stable bionanoconjugates were formed with logK in the range 6.8-8.9. An increase in enzyme activity was detected, in particular at acidic and close to neutral pH values, a feature that expands the useful pH range of the enzyme. A model for the adsorption was developed, based on geometrical considerations and volume increase data from dynamic light scattering. This indicates that enzymes adsorbed to gold nanoparticles are ca. 9 times more active than the free enzyme.",
author = "Almeida, {Miguel Peixoto de} and Pedro Quaresma and Susana Sousa and Cl{\'a}udia Couto and In{\^e}s Gomes and Ludwig Krippahl and Ricardo Franco and Eul{\'a}lia Pereira",
note = "Marta Giza is acknowledged for expert utilization of the UCSF Chimera package. Chimera is developed by the Resource for Biocomputing, Visualization, and Informatics at the University of California, San Francisco (supported by NIGMS P41-GM103311). This work was supported by (a) Unidade de Ciencias Biomoleculares Aplicadas-UCIBIO which is financed by Portuguese national funds from FCT/MEC (UID/Multi/04378/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728); and NOVA LINCS UID/CEC/04516/2013; and (b) European Union (FEDER funds through COMPETE) and National Funds (FCT, Fundacao para a Ciencia e Tecnologia), under the Partnership Agreement PT2020 through project UID/QUI/50006/2013-POCI/01/0145/FEDER/007265 (LAQV/REQUIMTE), Programa Operacional Regional do Norte (ON.2 - O Novo Norte), under the Quadro de Referencia Estrategico Nacional (QREN) and funded by Fundo Europeu de Desenvolvimento Regional NORTE-01-0145-FEDER-000011. PQ, SS and IG acknowledge their postdoctoral fellowships (SFRH/BPD/84018/2012, SFRH/BPD/103788/2014 and SFRH/BPD/63850/2009, respectively), and MPA acknowledges his doctoral fellowship (SFRH/BD/95983/2013); all fellowships are financed by Fundacao para a Ciencia e a Tecnologia, Portugal.",
year = "2018",
month = jan,
day = "1",
doi = "10.1039/c8cp03116a",
language = "English",
volume = "20",
pages = "16761--16769",
journal = "Physical Chemistry Chemical Physics",
issn = "1463-9076",
publisher = "RSC - Royal Society of Chemistry",
number = "24",
}