TY - JOUR
T1 - Mastadenovirus Molecular Diversity in Waste and Environmental Waters from the Lisbon Metropolitan Area
AU - Cavadas, Joana
AU - Parreira, Ricardo
AU - Leonardo, Inês
AU - Barreto Crespo, Maria Teresa
AU - Nunes, Mónica
N1 - Funding Information:
This work received financial support from the AgriWWater Project (PTDC/CTA-AMB/29586/2017), iNOVA4Health (UID/Multi/04462/2019), and Global Health and Tropical Medicine Center (which is funded through FCT contract UID/Multi/04413/2013).
Publisher Copyright:
© 2022 by the authors.
PY - 2022/12
Y1 - 2022/12
N2 - In face of the absence of epidemiological data regarding the circulation of human adenoviruses (HAdV) in Portugal, this study aimed at the evaluation of their molecular diversity in waste and environmental waters in the Lisbon Metropolitan Area (LMA). Using samples collected between 2018 and 2021, the HAdV hexon protein-coding sequence was partially amplified using three nested touch-down PCR protocols. The amplification products obtained were analyzed in parallel by two approaches: molecular cloning followed by Sanger sequencing and Next-Generation Sequencing (NGS) using Illumina® sequencing. The analysis of NGS-generated data allowed the identification of a higher diversity of HAdV-A (19%), -B (1%), -C (3%), -D (24%), and -F (25%) viral types, along with murine adenovirus (MAdV-2; 30%) in the wastewater treatment plant samples. On the other hand, HAdV-A (19%), -D (32%), and -F (36%) were identified in environmental samples, and possibly MAdV-2 (14%). These results demonstrate the presence of fecal contamination in environmental waters and the assessment of the diversity of this virus provides important information regarding the distribution of HAdV in LMA, including the detection of HAdV-F41, the most frequently reported in water worldwide.
AB - In face of the absence of epidemiological data regarding the circulation of human adenoviruses (HAdV) in Portugal, this study aimed at the evaluation of their molecular diversity in waste and environmental waters in the Lisbon Metropolitan Area (LMA). Using samples collected between 2018 and 2021, the HAdV hexon protein-coding sequence was partially amplified using three nested touch-down PCR protocols. The amplification products obtained were analyzed in parallel by two approaches: molecular cloning followed by Sanger sequencing and Next-Generation Sequencing (NGS) using Illumina® sequencing. The analysis of NGS-generated data allowed the identification of a higher diversity of HAdV-A (19%), -B (1%), -C (3%), -D (24%), and -F (25%) viral types, along with murine adenovirus (MAdV-2; 30%) in the wastewater treatment plant samples. On the other hand, HAdV-A (19%), -D (32%), and -F (36%) were identified in environmental samples, and possibly MAdV-2 (14%). These results demonstrate the presence of fecal contamination in environmental waters and the assessment of the diversity of this virus provides important information regarding the distribution of HAdV in LMA, including the detection of HAdV-F41, the most frequently reported in water worldwide.
KW - environmental waters
KW - human adenovirus
KW - molecular diversity
KW - nested PCR
KW - next-generation sequencing
KW - phylogenetic analysis
KW - wastewater
UR - http://www.scopus.com/inward/record.url?scp=85144731148&partnerID=8YFLogxK
U2 - 10.3390/microorganisms10122443
DO - 10.3390/microorganisms10122443
M3 - Article
AN - SCOPUS:85144731148
SN - 2076-2607
VL - 10
JO - Microorganisms
JF - Microorganisms
IS - 12
M1 - 2443
ER -
