Abstract
Metabolomics is a research field used to acquire comprehensive information on the composition of a metabolite pool to provide a functional screen of the cellular state. Studies of the plant metabolome include the analysis of a wide range of chemical species with very diverse physico-chemical properties, and therefore powerful analytical tools are required for the separation, characterization and quantification of this vast compound diversity present in plant matrices. In this review, challenges in the use ofmass spectrometry (MS) as a quantitative tool in plant metabolomics experiments are discussed, and important criteria for the development and validation of MS-based analytical methods provided.
| Original language | English |
|---|---|
| Article number | 20150370 |
| Journal | Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences |
| Volume | 374 |
| Issue number | 2079 |
| DOIs | |
| Publication status | Published - 28 Oct 2016 |
Keywords
- Analytical recoveries
- Mass spectrometry
- Matrix effects
- Metabolite quantification
- Method validation
- Plant metabolomics