TY - JOUR
T1 - Magnetic nanoparticles as a support for a copper (II) complex with nuclease activity
AU - Silva, Maria A. S.
AU - Romo, Adolfo I. B.
AU - Abreu, Dieric S.
AU - Carepo, Marta S. P.
AU - Lemus, Luis
AU - Jafelicci, Miguel
AU - Paulo, Tércio F.
AU - Nascimento, Otaciro R.
AU - Vargas, Esteban
AU - Denardin, Juliano C.
AU - Diógenes, Izaura C. N.
N1 - Sem PDF conforme despacho.
I. Diogenes (# 307078/2017-5), M. Jafelicci-Jr (# 309836/2013-1) and D. Abreu are thankful to CNPq and A. Romo (# 72170429) to CONICYT for the grants. E. Vargas and J. C. Denardin thanks to Basal FB0807. All authors are thankful to CNPq, CAPES, FUNCAP and FONDECYT (# 11130172) for financial support. The authors are grateful to Professor M. L. A. Temperini for the acquisition of Raman and SERS spectra at Sao Paulo University.
PY - 2018/9/1
Y1 - 2018/9/1
N2 - Magnetic nanoparticles have been extensively explored for the development of platforms for drug delivery and imaging probes. In this work, we have used a modular capping strategy to produce magnetic gold-coated Fe3O4 (Fe3O4@Au) nanoparticles, which have been decorated with a copper (II) complex containing a thioether derivative of clip-phen (Fe3O4@Au@Cu), where the complex [Cu(2CP-Bz-SMe)]2+ has affinity to bind DNA and proven nuclease activity (2CP-Bz-SMe=1,3-bis((1,10-phenanthrolin-2-yl)oxy)-N-(4-(methylthio)benzylidene)propan-2-imine). The functionalization of Fe3O4@Au with the copper complex occurs through the sulfur atom of the thioether moiety, as indicated by Raman scattering on surface. The magnetic measurements showed the nanomaterial Fe3O4@Au@Cu is still magnetic although the gold shell and the functionalization with the copper complex have diminished the magnetization due to the dilution of the magnetic core. The nuclease assays performed with Fe3O4@Au@Cu indicate that the nuclease activity of the nanomaterial toward the plasmid DNA involves an oxidative pathway in which H2O2 species is involved as intermediate in a Fenton-like reaction. Based on the electron paramagnetic resonance spectra (aN = 15.07 G, aH = 14.99 G), such nuclease activity is assigned, essentially, to the HO[rad] species indicating that the radical production property of [Cu(2CP-Bz-SMe)]2+ is successfully transferred to the core-shell gold-coated Fe3O4 magnetic nanoparticles. To the best of our knowledge, this is the first study reporting nuclease activity due to the reactive oxygen species generated by a copper complex immobilized on a gold-coated magnetic nanoparticle.
AB - Magnetic nanoparticles have been extensively explored for the development of platforms for drug delivery and imaging probes. In this work, we have used a modular capping strategy to produce magnetic gold-coated Fe3O4 (Fe3O4@Au) nanoparticles, which have been decorated with a copper (II) complex containing a thioether derivative of clip-phen (Fe3O4@Au@Cu), where the complex [Cu(2CP-Bz-SMe)]2+ has affinity to bind DNA and proven nuclease activity (2CP-Bz-SMe=1,3-bis((1,10-phenanthrolin-2-yl)oxy)-N-(4-(methylthio)benzylidene)propan-2-imine). The functionalization of Fe3O4@Au with the copper complex occurs through the sulfur atom of the thioether moiety, as indicated by Raman scattering on surface. The magnetic measurements showed the nanomaterial Fe3O4@Au@Cu is still magnetic although the gold shell and the functionalization with the copper complex have diminished the magnetization due to the dilution of the magnetic core. The nuclease assays performed with Fe3O4@Au@Cu indicate that the nuclease activity of the nanomaterial toward the plasmid DNA involves an oxidative pathway in which H2O2 species is involved as intermediate in a Fenton-like reaction. Based on the electron paramagnetic resonance spectra (aN = 15.07 G, aH = 14.99 G), such nuclease activity is assigned, essentially, to the HO[rad] species indicating that the radical production property of [Cu(2CP-Bz-SMe)]2+ is successfully transferred to the core-shell gold-coated Fe3O4 magnetic nanoparticles. To the best of our knowledge, this is the first study reporting nuclease activity due to the reactive oxygen species generated by a copper complex immobilized on a gold-coated magnetic nanoparticle.
KW - Copper complex
KW - Core-shell magnetic nanoparticles
KW - DNA nuclease activity
KW - Hydroxyl radical
KW - Reactive oxygen species
UR - http://www.scopus.com/inward/record.url?scp=85049478380&partnerID=8YFLogxK
U2 - 10.1016/j.jinorgbio.2018.06.015
DO - 10.1016/j.jinorgbio.2018.06.015
M3 - Article
C2 - 29996087
AN - SCOPUS:85049478380
SN - 0162-0134
VL - 186
SP - 294
EP - 300
JO - Journal of Inorganic Biochemistry
JF - Journal of Inorganic Biochemistry
ER -