Magnetic fishing of recombinant green fluorescent proteins and tagged proteins with designed synthetic ligands

Cláudia S.M. Fernandes; Ana Sofia Pina; Íris L. Batalha; A. Cecília A. Roque

doi:10.1080/01496395.2017.1375953

Magnetic fishing of recombinant green fluorescent proteins and tagged proteins with designed synthetic ligands

Cláudia S.M. Fernandes, Ana Sofia Pina, Íris L. Batalha, A. Cecília A. Roque

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Biomimetic ligands have emerged to overcome disadvantages inherent in biological ligands. In particular, the Ugi reaction can generate scaffolds where molecular diversity can be introduced, allowing the synthesis and screening of ligand libraries in a high-throughput manner against a variety of biological targets. Two adsorbents bearing Ugi-based synthetic ligands, coined A4C7 and A7C1, were previously developed for the selective recovery of green fluorescent protein (GFP) and RKRKRK-tagged GFP directly from Escherichia coli crude extracts. This work describes, for the first time, the in situ synthesis of Ugi-based ligands on magnetic beads and their application in the magnetic recovery of cognate proteins.

Original language	English
Pages (from-to)	2907-2915
Number of pages	9
Journal	Separation Science and Technology (Philadelphia)
Volume	52
Issue number	18
DOIs	https://doi.org/10.1080/01496395.2017.1375953
Publication status	Published - 12 Dec 2017

Keywords

GFP purification
magnetic fishing
magnetic particles
synthetic ligands
tagged-proteins purification

Access to Document

10.1080/01496395.2017.1375953

Cite this

@article{b0c624c090c54f9fb1cbfeb5588bd320,

title = "Magnetic fishing of recombinant green fluorescent proteins and tagged proteins with designed synthetic ligands",

abstract = "Biomimetic ligands have emerged to overcome disadvantages inherent in biological ligands. In particular, the Ugi reaction can generate scaffolds where molecular diversity can be introduced, allowing the synthesis and screening of ligand libraries in a high-throughput manner against a variety of biological targets. Two adsorbents bearing Ugi-based synthetic ligands, coined A4C7 and A7C1, were previously developed for the selective recovery of green fluorescent protein (GFP) and RKRKRK-tagged GFP directly from Escherichia coli crude extracts. This work describes, for the first time, the in situ synthesis of Ugi-based ligands on magnetic beads and their application in the magnetic recovery of cognate proteins.",

keywords = "GFP purification, magnetic fishing, magnetic particles, synthetic ligands, tagged-proteins purification",

author = "Fernandes, {Cl{\'a}udia S.M.} and Pina, {Ana Sofia} and Batalha, {{\'I}ris L.} and Roque, {A. Cec{\'i}lia A.}",

note = "sem pdf conforme despacho. Unidade de Ciencias Biomoleculares Aplicadas-UCIBIO - FCT/MEC UID/Multi/04378/2013 ; ERDF : POCI-01-0145-FEDER-007728 ; PTDC/EBB-BIO/118317/2010; SFRH/BPD/97585/2013 ; SFRH/BD/64427/2009 ; PD/BD/105871/2014 ",

year = "2017",

month = dec,

day = "12",

doi = "10.1080/01496395.2017.1375953",

language = "English",

volume = "52",

pages = "2907--2915",

journal = "Separation Science and Technology (Philadelphia)",

issn = "0149-6395",

publisher = "Taylor & Francis",

number = "18",

}

TY - JOUR

T1 - Magnetic fishing of recombinant green fluorescent proteins and tagged proteins with designed synthetic ligands

AU - Fernandes, Cláudia S.M.

AU - Pina, Ana Sofia

AU - Batalha, Íris L.

AU - Roque, A. Cecília A.

N1 - sem pdf conforme despacho. Unidade de Ciencias Biomoleculares Aplicadas-UCIBIO - FCT/MEC UID/Multi/04378/2013 ; ERDF : POCI-01-0145-FEDER-007728 ; PTDC/EBB-BIO/118317/2010; SFRH/BPD/97585/2013 ; SFRH/BD/64427/2009 ; PD/BD/105871/2014

PY - 2017/12/12

Y1 - 2017/12/12

N2 - Biomimetic ligands have emerged to overcome disadvantages inherent in biological ligands. In particular, the Ugi reaction can generate scaffolds where molecular diversity can be introduced, allowing the synthesis and screening of ligand libraries in a high-throughput manner against a variety of biological targets. Two adsorbents bearing Ugi-based synthetic ligands, coined A4C7 and A7C1, were previously developed for the selective recovery of green fluorescent protein (GFP) and RKRKRK-tagged GFP directly from Escherichia coli crude extracts. This work describes, for the first time, the in situ synthesis of Ugi-based ligands on magnetic beads and their application in the magnetic recovery of cognate proteins.

AB - Biomimetic ligands have emerged to overcome disadvantages inherent in biological ligands. In particular, the Ugi reaction can generate scaffolds where molecular diversity can be introduced, allowing the synthesis and screening of ligand libraries in a high-throughput manner against a variety of biological targets. Two adsorbents bearing Ugi-based synthetic ligands, coined A4C7 and A7C1, were previously developed for the selective recovery of green fluorescent protein (GFP) and RKRKRK-tagged GFP directly from Escherichia coli crude extracts. This work describes, for the first time, the in situ synthesis of Ugi-based ligands on magnetic beads and their application in the magnetic recovery of cognate proteins.

KW - GFP purification

KW - magnetic fishing

KW - magnetic particles

KW - synthetic ligands

KW - tagged-proteins purification

UR - http://www.scopus.com/inward/record.url?scp=85032210145&partnerID=8YFLogxK

U2 - 10.1080/01496395.2017.1375953

DO - 10.1080/01496395.2017.1375953

M3 - Article

AN - SCOPUS:85032210145

SN - 0149-6395

VL - 52

SP - 2907

EP - 2915

JO - Separation Science and Technology (Philadelphia)

JF - Separation Science and Technology (Philadelphia)

IS - 18

ER -

Magnetic fishing of recombinant green fluorescent proteins and tagged proteins with designed synthetic ligands

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this