M13 bacteriophage purification using poly(ionic liquids) as alternative separation matrices

Maria João Jacinto, David J.S. Patinha, Isabel M. Marrucho, João Gonçalves, Richard C. Willson, Ana M. Azevedo, M. Raquel Aires-Barros

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

M13 is a filamentous, non-lytic bacteriophage that infects Escherichia coli via the F pilus. Currently, phage M13 is widely used in phage display technology and bio-nanotechnology, and is considered a possible antibacterial therapeutic agent, among other applications. Conventional phage purification involves 5–7 operational steps, with high operational costs and significant product loss (approximately 60%). In this work, we propose a scalable purification process for M13 bacteriophage using a novel stationary phase based on a polymeric ionic liquid (PIL) with a positively charged backbone structure. Poly (1–vinyl-3-ethyl imidazolium bis(trifluoromethylsulfonyl) imide) - poly(VEIM-TFSI) predominantly acted as an anion exchanger under binding-elution mode. This revealed to be a rapid and simple method for the recovery of phage M13 with an overall separation yield of over 70% after a single downstream step. To the best of our knowledge, PILs have never been used as separation matrices for biological products and the results obtained, together with the large number of cations and anions available to prepare PILs, illustrate well the large potential of the proposed methodology.

Original languageEnglish
Pages (from-to)246-250
Number of pages5
JournalJournal Of Chromatography A
Volume1532
DOIs
Publication statusPublished - 12 Jan 2018

Keywords

  • Anion exchange
  • Batch adsorption
  • Downstream processing
  • M13 bacteriophage
  • Poly(ionic liquids)

Fingerprint Dive into the research topics of 'M13 bacteriophage purification using poly(ionic liquids) as alternative separation matrices'. Together they form a unique fingerprint.

  • Cite this