Localized surface plasmon resonance (LSPR) biosensing using gold nanotriangles

Detection of DNA hybridization events at room temperature

Leonor Soares, Andrea Csáki, Jacqueline Jatschka, Wolfgang Fritzsche, Orfeu Flores, Ricardo Franco, Eulália Pereira

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

We present a proof-of-concept of the application of gold nanotriangles in sequence specific DNA detection, using localized surface plasmon resonance (LSPR) spectroscopy and dark-field optical microscopy. The sensing platform comprises gold nanotriangles immobilized on a glass chip and oligonucleotides as probes. Probe formation and testing complementary and non-complementary targets followed common chip technology protocols. Gold nanotriangles showed a remarkable sensitivity of 468 nm per RIU and allowed detection of 20-mer targets. When the target sequence was part of a 50-mer synthetic DNA oligonucleotide, LSPR shifts as high as 35 nm were observed. Conversely, when the target was present in PCR products of ca. 350 bp, obtained from clinical samples, LSPR shifts larger than 20 nm were observed. Moreover, LSPR shifts were less than ±1 nm for the respective non-complementary targets. These results with gold nanotriangles as sensors are a notable improvement to the LSPR shifts of less than 5 nm usually obtained for spherical gold nanoparticles of comparable sizes. Optimal conditions for the detection of synthetic and PCR product targets using gold nanotriangles and oligonucleotide probes were achieved with low percentages of intercalating thioalkanes; target hybridization at room temperature, 3 hours of incubation, and 2× SSC buffer stringency conditions.

Original languageEnglish
Pages (from-to)4964-4973
Number of pages10
JournalAnalyst
Volume139
Issue number19
DOIs
Publication statusPublished - 2014

Fingerprint

Surface Plasmon Resonance
Surface plasmon resonance
Gold
DNA
gold
Temperature
Oligonucleotides
Oligonucleotide Probes
temperature
probe
Polymerase Chain Reaction
Nanoparticles
Optical microscopy
Glass
detection
microscopy
Microscopy
Spectrum Analysis
Buffers
glass

Keywords

  • NOBLE-METAL NANOPARTICLES
  • BIOMEDICAL APPLICATIONS
  • SILVER NANOPARTICLES
  • SENSITIVITY
  • NANOSTRUCTURES
  • DIAGNOSTICS
  • NANOPRISMS
  • EXPRESSION
  • MONOLAYERS
  • LAYER

Cite this

Soares, Leonor ; Csáki, Andrea ; Jatschka, Jacqueline ; Fritzsche, Wolfgang ; Flores, Orfeu ; Franco, Ricardo ; Pereira, Eulália. / Localized surface plasmon resonance (LSPR) biosensing using gold nanotriangles : Detection of DNA hybridization events at room temperature. In: Analyst. 2014 ; Vol. 139, No. 19. pp. 4964-4973.
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Localized surface plasmon resonance (LSPR) biosensing using gold nanotriangles : Detection of DNA hybridization events at room temperature. / Soares, Leonor; Csáki, Andrea; Jatschka, Jacqueline; Fritzsche, Wolfgang; Flores, Orfeu; Franco, Ricardo; Pereira, Eulália.

In: Analyst, Vol. 139, No. 19, 2014, p. 4964-4973.

Research output: Contribution to journalArticle

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T1 - Localized surface plasmon resonance (LSPR) biosensing using gold nanotriangles

T2 - Detection of DNA hybridization events at room temperature

AU - Soares, Leonor

AU - Csáki, Andrea

AU - Jatschka, Jacqueline

AU - Fritzsche, Wolfgang

AU - Flores, Orfeu

AU - Franco, Ricardo

AU - Pereira, Eulália

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AB - We present a proof-of-concept of the application of gold nanotriangles in sequence specific DNA detection, using localized surface plasmon resonance (LSPR) spectroscopy and dark-field optical microscopy. The sensing platform comprises gold nanotriangles immobilized on a glass chip and oligonucleotides as probes. Probe formation and testing complementary and non-complementary targets followed common chip technology protocols. Gold nanotriangles showed a remarkable sensitivity of 468 nm per RIU and allowed detection of 20-mer targets. When the target sequence was part of a 50-mer synthetic DNA oligonucleotide, LSPR shifts as high as 35 nm were observed. Conversely, when the target was present in PCR products of ca. 350 bp, obtained from clinical samples, LSPR shifts larger than 20 nm were observed. Moreover, LSPR shifts were less than ±1 nm for the respective non-complementary targets. These results with gold nanotriangles as sensors are a notable improvement to the LSPR shifts of less than 5 nm usually obtained for spherical gold nanoparticles of comparable sizes. Optimal conditions for the detection of synthetic and PCR product targets using gold nanotriangles and oligonucleotide probes were achieved with low percentages of intercalating thioalkanes; target hybridization at room temperature, 3 hours of incubation, and 2× SSC buffer stringency conditions.

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KW - DIAGNOSTICS

KW - NANOPRISMS

KW - EXPRESSION

KW - MONOLAYERS

KW - LAYER

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SN - 0003-2654

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