Liquid chromatographic and electrophoretic characterisation of extracellular β-glucosidase of Pleurotus ostreatus grown in organic waste

Helena Morais, Cristina Ramos, Natalia Matos, Esther Forgács, Tibor Cserháti, Vitor Almeida, José Oliveira, Yassin Darwish, Zoltán Illés

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

The production of β-glucosidase by the ligninolytic fungus Pleurotus ostreatus has been studied in different culture media containing agro-industrial wastes. The enzyme is purified by anion-exchange chromatography, the molecular mass and isoelectric point of purified β-glucosidase are measured by sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and isoelectric focusing and the stability and kinetic parameters of the enzyme assessed by spectrophotometry. It has been established that the retention time, molecular mass and isoelectric point of the enzyme depend on the composition of the culture media while the activity and stability of β-glucosidases of different origin were very similar. The combined chromatographic and electrophoretic methods have proved to be suitable techniques for the purification and characterisation of the β-glucosidases produced by the ligninolytic fungus Pleurotus ostreatus in different culture media.
Original languageEnglish
Pages (from-to)111-119
Number of pages9
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume770
Issue number1-2
DOIs
Publication statusPublished - 25 Apr 2002

Keywords

  • β-Glucosidase
  • Enzymes
  • Pleurotus ostreatus

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