Lipoperoxidation products and thiol antioxidants in chromium exposed workers

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Hexavalent chromium is an established carcinogenic agent, which is not directly reactive with DNA. Its genotoxicity involves a reduction step, producing reactive oxygen species and radicals, and also lower valence forms which form stable complexes with intracellular macromolecules. The trivalent form of chromium may directly react with the genetic material and has also been shown to generate oxidative damage in vitro. To further evaluate the importance of in vivo oxidative DNA damage in the toxicity of each valence form, we conducted a comparative study on hexavalent and trivalent chromium-exposed workers (manual metal arc stainless steel welders and leather tanning workers), focusing on the total oxidative status by quantifying the level of lipoperoxidation products in urine. Thiol antioxidants are important in response to oxidative stress, and therefore, the concentration of glutathione and cysteine in peripheral blood lymphocytes was also determined. Chromium exposure was evaluated by quantifying total chromium in plasma and urine. Both groups had a signficant increase in lipid peroxidation products expressed as malondialdehyde (MDA) in urine (tanners 1.42 ± 0.61 μmol/g creatinine, welders 1.67 ± 1.13 μmol/g creatinine versus controls 0.81 ± 0.26 μmol/g creatinine, P < 0.005 in both cases) but only welders had a significant decrease in glutathione concentration in lymphocytes. There was a positive correlation between chromium in plasma and urinary MDA in welders, but not in tanners. This work is part of a larger study of which major results have been published previously including cytogenetics and DNA-protein cross-links in workers exposed to the two different forms of chromium. These results are compared with the results of oxidative damage from this study. © The Author 2005. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society.
Original languageEnglish
Pages (from-to)311-315
Number of pages5
JournalMutagenesis
Volume20
Issue number5
DOIs
Publication statusPublished - Sep 2005

Fingerprint

Chromium
Sulfhydryl Compounds
Antioxidants
Creatinine
Urine
Malondialdehyde
Lymphocytes
Glutathione
Reactive Oxygen Species
DNA
Tanning
Stainless Steel
Plasmas
Mutagens
Oxidative stress
Leather
Cytogenetics
Lipid Peroxidation
DNA Damage
Cysteine

Keywords

  • stainless steel
  • thiol
  • chromium
  • creatinine
  • cysteine
  • DNA
  • glutathione
  • malonaldehyde
  • metal
  • reactive oxygen metabolite

Cite this

@article{9f61a73f3e2c4214bed7968c1760457b,
title = "Lipoperoxidation products and thiol antioxidants in chromium exposed workers",
abstract = "Hexavalent chromium is an established carcinogenic agent, which is not directly reactive with DNA. Its genotoxicity involves a reduction step, producing reactive oxygen species and radicals, and also lower valence forms which form stable complexes with intracellular macromolecules. The trivalent form of chromium may directly react with the genetic material and has also been shown to generate oxidative damage in vitro. To further evaluate the importance of in vivo oxidative DNA damage in the toxicity of each valence form, we conducted a comparative study on hexavalent and trivalent chromium-exposed workers (manual metal arc stainless steel welders and leather tanning workers), focusing on the total oxidative status by quantifying the level of lipoperoxidation products in urine. Thiol antioxidants are important in response to oxidative stress, and therefore, the concentration of glutathione and cysteine in peripheral blood lymphocytes was also determined. Chromium exposure was evaluated by quantifying total chromium in plasma and urine. Both groups had a signficant increase in lipid peroxidation products expressed as malondialdehyde (MDA) in urine (tanners 1.42 ± 0.61 μmol/g creatinine, welders 1.67 ± 1.13 μmol/g creatinine versus controls 0.81 ± 0.26 μmol/g creatinine, P < 0.005 in both cases) but only welders had a significant decrease in glutathione concentration in lymphocytes. There was a positive correlation between chromium in plasma and urinary MDA in welders, but not in tanners. This work is part of a larger study of which major results have been published previously including cytogenetics and DNA-protein cross-links in workers exposed to the two different forms of chromium. These results are compared with the results of oxidative damage from this study. {\circledC} The Author 2005. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society.",
keywords = "stainless steel, thiol, chromium, creatinine, cysteine, DNA, glutathione, malonaldehyde, metal, reactive oxygen metabolite",
author = "Margarida Goulart and Bator{\'e}u, {Maria Camila} and Rodrigues, {Ant{\'o}nio Sebasti{\~a}o} and Laires, {Ant{\'o}nio Jos{\'e} Cabrita Lucas} and J. Rueff",
year = "2005",
month = "9",
doi = "10.1093/mutage/gei043",
language = "English",
volume = "20",
pages = "311--315",
journal = "Mutagenesis",
issn = "0267-8357",
publisher = "OXFORD UNIV PRESS INC",
number = "5",

}

Lipoperoxidation products and thiol antioxidants in chromium exposed workers. / Goulart, Margarida; Batoréu, Maria Camila; Rodrigues, António Sebastião; Laires, António José Cabrita Lucas; Rueff, J.

In: Mutagenesis, Vol. 20, No. 5, 09.2005, p. 311-315.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Lipoperoxidation products and thiol antioxidants in chromium exposed workers

AU - Goulart, Margarida

AU - Batoréu, Maria Camila

AU - Rodrigues, António Sebastião

AU - Laires, António José Cabrita Lucas

AU - Rueff, J.

PY - 2005/9

Y1 - 2005/9

N2 - Hexavalent chromium is an established carcinogenic agent, which is not directly reactive with DNA. Its genotoxicity involves a reduction step, producing reactive oxygen species and radicals, and also lower valence forms which form stable complexes with intracellular macromolecules. The trivalent form of chromium may directly react with the genetic material and has also been shown to generate oxidative damage in vitro. To further evaluate the importance of in vivo oxidative DNA damage in the toxicity of each valence form, we conducted a comparative study on hexavalent and trivalent chromium-exposed workers (manual metal arc stainless steel welders and leather tanning workers), focusing on the total oxidative status by quantifying the level of lipoperoxidation products in urine. Thiol antioxidants are important in response to oxidative stress, and therefore, the concentration of glutathione and cysteine in peripheral blood lymphocytes was also determined. Chromium exposure was evaluated by quantifying total chromium in plasma and urine. Both groups had a signficant increase in lipid peroxidation products expressed as malondialdehyde (MDA) in urine (tanners 1.42 ± 0.61 μmol/g creatinine, welders 1.67 ± 1.13 μmol/g creatinine versus controls 0.81 ± 0.26 μmol/g creatinine, P < 0.005 in both cases) but only welders had a significant decrease in glutathione concentration in lymphocytes. There was a positive correlation between chromium in plasma and urinary MDA in welders, but not in tanners. This work is part of a larger study of which major results have been published previously including cytogenetics and DNA-protein cross-links in workers exposed to the two different forms of chromium. These results are compared with the results of oxidative damage from this study. © The Author 2005. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society.

AB - Hexavalent chromium is an established carcinogenic agent, which is not directly reactive with DNA. Its genotoxicity involves a reduction step, producing reactive oxygen species and radicals, and also lower valence forms which form stable complexes with intracellular macromolecules. The trivalent form of chromium may directly react with the genetic material and has also been shown to generate oxidative damage in vitro. To further evaluate the importance of in vivo oxidative DNA damage in the toxicity of each valence form, we conducted a comparative study on hexavalent and trivalent chromium-exposed workers (manual metal arc stainless steel welders and leather tanning workers), focusing on the total oxidative status by quantifying the level of lipoperoxidation products in urine. Thiol antioxidants are important in response to oxidative stress, and therefore, the concentration of glutathione and cysteine in peripheral blood lymphocytes was also determined. Chromium exposure was evaluated by quantifying total chromium in plasma and urine. Both groups had a signficant increase in lipid peroxidation products expressed as malondialdehyde (MDA) in urine (tanners 1.42 ± 0.61 μmol/g creatinine, welders 1.67 ± 1.13 μmol/g creatinine versus controls 0.81 ± 0.26 μmol/g creatinine, P < 0.005 in both cases) but only welders had a significant decrease in glutathione concentration in lymphocytes. There was a positive correlation between chromium in plasma and urinary MDA in welders, but not in tanners. This work is part of a larger study of which major results have been published previously including cytogenetics and DNA-protein cross-links in workers exposed to the two different forms of chromium. These results are compared with the results of oxidative damage from this study. © The Author 2005. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society.

KW - stainless steel

KW - thiol

KW - chromium

KW - creatinine

KW - cysteine

KW - DNA

KW - glutathione

KW - malonaldehyde

KW - metal

KW - reactive oxygen metabolite

UR - http://www.scopus.com/record/display.uri?eid=2-s2.0-24044544600&origin=resultslist&sort=plf-f&src=s&st1

U2 - 10.1093/mutage/gei043

DO - 10.1093/mutage/gei043

M3 - Article

VL - 20

SP - 311

EP - 315

JO - Mutagenesis

JF - Mutagenesis

SN - 0267-8357

IS - 5

ER -