Lipase-catalyzed consecutive batch reaction for production of steryl esters from vegetable oil deodorizer distillates

A. R S Teixeira, J. L C Santos, J. G. Crespo

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A significant decrease of the yield of steryl esters production via the esterification of sterols and free fatty acids (FFA) present in vegetable oil deodorizer distillates was observed when consecutive batch reactions (24 hr each) were carried out using the same enzyme and under optimal operating conditions. The objective of this work is to identify the causes of such phenomena, in order to avoid or minimize it, allowing for reuse of the Candida rugosa lipase catalyst in consecutive batch reactions. The effect of water activity and glycerol produced by hydrolysis of acylglycerides, on the stability of the enzyme was studied. Results show that these effects were not the main reason for the decrease of yield in consecutive batch reactions. The presence of oxidation products proved to play an important role in the enzyme inactivation. Because of the high concentration of antioxidants (phenolic compounds, squalene, and tocopherols) naturally present in deodorizer distillates, the inactivation of the enzyme was minimized; nevertheless, it could not be avoided. A partial reuse of the enzyme is technically a possible solution, by adding fresh enzyme in the beginning of each batch reaction. Four consecutive batch reactions (24 hr each) were successfully carried out under optimal conditions, keeping a constant yield of steryl esters (SE) production (>85%).

Original languageEnglish
Pages (from-to)5443-5455
Number of pages13
JournalIndustrial & Engineering Chemistry Research
Volume51
Issue number15
DOIs
Publication statusPublished - 18 Apr 2012

Fingerprint

Plant Oils
Vegetable oils
Lipases
Lipase
Esters
Enzymes
Squalene
Tocopherols
Candida
Esterification
Sterols
Antioxidants
Glycerol
Fatty acids
Nonesterified Fatty Acids
Hydrolysis
Oxidation
Catalysts
Water

Keywords

  • ENZYMATIC TRANSESTERIFICATION
  • CANDIDA-RUGOSA
  • PHYTOSTEROLS
  • INTERESTERIFICATION
  • FATS

Cite this

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title = "Lipase-catalyzed consecutive batch reaction for production of steryl esters from vegetable oil deodorizer distillates",
abstract = "A significant decrease of the yield of steryl esters production via the esterification of sterols and free fatty acids (FFA) present in vegetable oil deodorizer distillates was observed when consecutive batch reactions (24 hr each) were carried out using the same enzyme and under optimal operating conditions. The objective of this work is to identify the causes of such phenomena, in order to avoid or minimize it, allowing for reuse of the Candida rugosa lipase catalyst in consecutive batch reactions. The effect of water activity and glycerol produced by hydrolysis of acylglycerides, on the stability of the enzyme was studied. Results show that these effects were not the main reason for the decrease of yield in consecutive batch reactions. The presence of oxidation products proved to play an important role in the enzyme inactivation. Because of the high concentration of antioxidants (phenolic compounds, squalene, and tocopherols) naturally present in deodorizer distillates, the inactivation of the enzyme was minimized; nevertheless, it could not be avoided. A partial reuse of the enzyme is technically a possible solution, by adding fresh enzyme in the beginning of each batch reaction. Four consecutive batch reactions (24 hr each) were successfully carried out under optimal conditions, keeping a constant yield of steryl esters (SE) production (>85{\%}).",
keywords = "ENZYMATIC TRANSESTERIFICATION, CANDIDA-RUGOSA, PHYTOSTEROLS, INTERESTERIFICATION, FATS",
author = "Teixeira, {A. R S} and Santos, {J. L C} and Crespo, {J. G.}",
note = "A.R.S.T. acknowledges the financial support of Fundacao para a Ciencia e a Tecnologia (through Ph.D. Research Grant No. SFRH/BD/46023/2008). The authors acknowledge the financial support of the European Commission through Project OPTIM'OILS (Contract No. FP6-2005-FOOD 36318). The authors also acknowledge the samples of deodorizer distillates provided by Lesieur (France) and Lesieur Crystal (Morocco).",
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Lipase-catalyzed consecutive batch reaction for production of steryl esters from vegetable oil deodorizer distillates. / Teixeira, A. R S; Santos, J. L C; Crespo, J. G.

In: Industrial & Engineering Chemistry Research, Vol. 51, No. 15, 18.04.2012, p. 5443-5455.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Lipase-catalyzed consecutive batch reaction for production of steryl esters from vegetable oil deodorizer distillates

AU - Teixeira, A. R S

AU - Santos, J. L C

AU - Crespo, J. G.

N1 - A.R.S.T. acknowledges the financial support of Fundacao para a Ciencia e a Tecnologia (through Ph.D. Research Grant No. SFRH/BD/46023/2008). The authors acknowledge the financial support of the European Commission through Project OPTIM'OILS (Contract No. FP6-2005-FOOD 36318). The authors also acknowledge the samples of deodorizer distillates provided by Lesieur (France) and Lesieur Crystal (Morocco).

PY - 2012/4/18

Y1 - 2012/4/18

N2 - A significant decrease of the yield of steryl esters production via the esterification of sterols and free fatty acids (FFA) present in vegetable oil deodorizer distillates was observed when consecutive batch reactions (24 hr each) were carried out using the same enzyme and under optimal operating conditions. The objective of this work is to identify the causes of such phenomena, in order to avoid or minimize it, allowing for reuse of the Candida rugosa lipase catalyst in consecutive batch reactions. The effect of water activity and glycerol produced by hydrolysis of acylglycerides, on the stability of the enzyme was studied. Results show that these effects were not the main reason for the decrease of yield in consecutive batch reactions. The presence of oxidation products proved to play an important role in the enzyme inactivation. Because of the high concentration of antioxidants (phenolic compounds, squalene, and tocopherols) naturally present in deodorizer distillates, the inactivation of the enzyme was minimized; nevertheless, it could not be avoided. A partial reuse of the enzyme is technically a possible solution, by adding fresh enzyme in the beginning of each batch reaction. Four consecutive batch reactions (24 hr each) were successfully carried out under optimal conditions, keeping a constant yield of steryl esters (SE) production (>85%).

AB - A significant decrease of the yield of steryl esters production via the esterification of sterols and free fatty acids (FFA) present in vegetable oil deodorizer distillates was observed when consecutive batch reactions (24 hr each) were carried out using the same enzyme and under optimal operating conditions. The objective of this work is to identify the causes of such phenomena, in order to avoid or minimize it, allowing for reuse of the Candida rugosa lipase catalyst in consecutive batch reactions. The effect of water activity and glycerol produced by hydrolysis of acylglycerides, on the stability of the enzyme was studied. Results show that these effects were not the main reason for the decrease of yield in consecutive batch reactions. The presence of oxidation products proved to play an important role in the enzyme inactivation. Because of the high concentration of antioxidants (phenolic compounds, squalene, and tocopherols) naturally present in deodorizer distillates, the inactivation of the enzyme was minimized; nevertheless, it could not be avoided. A partial reuse of the enzyme is technically a possible solution, by adding fresh enzyme in the beginning of each batch reaction. Four consecutive batch reactions (24 hr each) were successfully carried out under optimal conditions, keeping a constant yield of steryl esters (SE) production (>85%).

KW - ENZYMATIC TRANSESTERIFICATION

KW - CANDIDA-RUGOSA

KW - PHYTOSTEROLS

KW - INTERESTERIFICATION

KW - FATS

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DO - 10.1021/ie202853f

M3 - Article

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SP - 5443

EP - 5455

JO - Industrial & Engineering Chemistry Research

JF - Industrial & Engineering Chemistry Research

SN - 0888-5885

IS - 15

ER -