TY - JOUR
T1 - Leishmania infantum released proteins specifically regulate cytokine expression and production patterns by CD4+ and CD8+ T cells
AU - Rosa, Ricardo
AU - Marques, Cláudia
AU - Rodrigues, Olivia Roos
AU - Santos-Gomes, Gabriela M.
N1 - Funding Information:
The authors thank J.M. Cristóvão and J. Ramada for technical assistance. Funding for this work was provided by the project research grant POCTI/CVT/37759/2001 by the Fundação para a Ciência e Tecnologia (FCT), Portugal partially supported by the European Union Fund (FEDER), and grant SFRH/BD/997/2000 from FCT.
PY - 2006/3
Y1 - 2006/3
N2 - Specific immune responses by CD4+ and CD8+ T cells, from two infected mice strains (BALB/c and C57BL/6), induced by High, Inter and Low protein fractions released by Leishmania infantum, were assessed through the evaluation of IL-12, IFN-γ and IL-10 mRNA by real-time PCR and respective protein production by ELISA. During infection establishment, High and Inter fractions directed both mice strains T cells subsets to increase the production of IFN-γ, associated to IL-12 release. Later on, parasite replication augmented in BALB/c and stabilised in C57BL/6 mice. Inter fraction induced CD4+ T cells to maintain IFN-γ production, with the simultaneous release of IL-12 by both cell subsets in BALB/c mice and by CD8+ T cells in C57BL/6 mice. These observations suggested a prophylactic potential for Inter fraction which was able to induce Th1 response with IL-12 involvement, required for the maintenance of memory cells, in mice strains with different parasitic evolution.
AB - Specific immune responses by CD4+ and CD8+ T cells, from two infected mice strains (BALB/c and C57BL/6), induced by High, Inter and Low protein fractions released by Leishmania infantum, were assessed through the evaluation of IL-12, IFN-γ and IL-10 mRNA by real-time PCR and respective protein production by ELISA. During infection establishment, High and Inter fractions directed both mice strains T cells subsets to increase the production of IFN-γ, associated to IL-12 release. Later on, parasite replication augmented in BALB/c and stabilised in C57BL/6 mice. Inter fraction induced CD4+ T cells to maintain IFN-γ production, with the simultaneous release of IL-12 by both cell subsets in BALB/c mice and by CD8+ T cells in C57BL/6 mice. These observations suggested a prophylactic potential for Inter fraction which was able to induce Th1 response with IL-12 involvement, required for the maintenance of memory cells, in mice strains with different parasitic evolution.
KW - CD4 T cells
KW - CD8 T cells
KW - Cytokine
KW - L. infantum released proteins
KW - Rodent model
UR - http://www.scopus.com/inward/record.url?scp=33644519971&partnerID=8YFLogxK
U2 - 10.1016/j.actatropica.2005.12.003
DO - 10.1016/j.actatropica.2005.12.003
M3 - Article
C2 - 16442069
AN - SCOPUS:33644519971
SN - 0001-706X
VL - 97
SP - 309
EP - 317
JO - Acta Tropica
JF - Acta Tropica
IS - 3
ER -
