TY - JOUR
T1 - LC-DAD-ESI-MS/MS analysis and cytotoxic and antiproliferative effects of chlorogenic acid derivative rich extract from
T2 - Nerium oleander L. pink flowers
AU - Ayouaz, Siham
AU - Oliveira-Alves, Sheila Cristina
AU - Serra, Ana Teresa
AU - Lefsih, Khalef
AU - Samah, Madani
AU - Bento Da Silva, Andreia
AU - Madani, Khodir
AU - Bronze, Maria Rosário
N1 - Funding Information:
Funding sources: This study was funded and supported by FCT, Portuguese Mass Spectrometry Network (Rede Nacional de Espectrometria de Massa – RNEM; LISBOA-01-0145-FEDER-402-022125), iNOVA4Health—UID/Multi/04462/2020 and INTERFACE Programme (Innovation, Technology and Circular Economy Fund (FITEC)). ATS also acknowledge Fundação para a Ciência e Tecnologia/Ministério da Educação e Ciência for the Individual Grant CEECIND/04801/2017. The authors are grateful to iMed and iBET for their great help.
Publisher Copyright:
© The Royal Society of Chemistry.
PY - 2021/4/21
Y1 - 2021/4/21
N2 - Nerium oleander L. is a widely used medicinal plant for pharmaceutical purposes. In this work, an extract of the pink flowers of this plant (FE) was characterized in terms of phenolic composition by LC-DAD-ESI-MS/MS and bioactivity, namely, antioxidant and antiproliferative effects. A total of 20 compounds from different classes, including derivatives of phenolic acids and flavonoid glycosylated derivatives, were identified in FE. Chlorogenic acid was the dominant phenolic compound in the extract (62.28 ± 1.74 μg mg-1 of dry extract). The antioxidant activity was assessed by ORAC assay, and FE showed an ability to reduce peroxyl radicals (ORAC value of 791.26 μmol TEAC per g DE). Additionally, the FE inhibited the proliferation of a colorectal cancer cell line (HT29 cells, EC50 = 11.72 ± 0.02 μg mL-1) and showed no cytotoxicity to confluent Caco-2 cells, a model of human intestinal epithelium. These results provide new information about the phenolic composition of Nerium oleander pink flowers and the bioactivity of the extracts.
AB - Nerium oleander L. is a widely used medicinal plant for pharmaceutical purposes. In this work, an extract of the pink flowers of this plant (FE) was characterized in terms of phenolic composition by LC-DAD-ESI-MS/MS and bioactivity, namely, antioxidant and antiproliferative effects. A total of 20 compounds from different classes, including derivatives of phenolic acids and flavonoid glycosylated derivatives, were identified in FE. Chlorogenic acid was the dominant phenolic compound in the extract (62.28 ± 1.74 μg mg-1 of dry extract). The antioxidant activity was assessed by ORAC assay, and FE showed an ability to reduce peroxyl radicals (ORAC value of 791.26 μmol TEAC per g DE). Additionally, the FE inhibited the proliferation of a colorectal cancer cell line (HT29 cells, EC50 = 11.72 ± 0.02 μg mL-1) and showed no cytotoxicity to confluent Caco-2 cells, a model of human intestinal epithelium. These results provide new information about the phenolic composition of Nerium oleander pink flowers and the bioactivity of the extracts.
UR - http://www.scopus.com/inward/record.url?scp=85104982996&partnerID=8YFLogxK
U2 - 10.1039/d0fo02640a
DO - 10.1039/d0fo02640a
M3 - Article
C2 - 33900304
AN - SCOPUS:85104982996
SN - 2042-6496
VL - 12
SP - 3624
EP - 3634
JO - Food and Function
JF - Food and Function
IS - 8
ER -
