Laser ablation and inductively coupled plasma mass spectrometry focusing on bioimaging from elemental distribution using MatLab software

A practical guide

Gustavo S. Pessôa, José L. Capelo-Martínez, Florentino Fdez-Riverola, Hugo López-Fernández, Daniel Glez-Peña, Miguel Reboiro-Jato, Marco A. Z. Arruda

Research output: Contribution to journalReview article

12 Citations (Scopus)

Abstract

The parameters influencing laser ablation inductively coupled plasma mass spectrometry as a tool for imaging elemental distribution in tissues are critically commented on in this work, and penile cancer tissue was used as a model. General aspects of LA-ICP-MS are discussed, and among them, issues regarding sample preparation and calibration. The optimization process of the following variables is described in detail in a step-by-step tutorial manner: laser intensity, laser frequency, laser resolution, ICP radiofrequency, nebulizer and auxiliary gas flow rates, and C isotopes as internal standards. Once the best condition is found for each variable, MATLAB software is used to generate two-dimensional images. Herein it is also explained how to use MATLAB software to generate tissue images using acquisition, exporting and data treatment parameters.

Original languageEnglish
Pages (from-to)832-840
Number of pages9
JournalJournal of Analytical Atomic Spectrometry
Volume31
Issue number4
DOIs
Publication statusPublished - 1 Apr 2016

Fingerprint

Inductively coupled plasma mass spectrometry
Laser ablation
Tissue
MATLAB
Lasers
Image acquisition
Isotopes
Flow of gases
Flow rate
Calibration
Imaging techniques

Keywords

  • LA-ICP-MS
  • SOLUTION-BASED CALIBRATION
  • BIOLOGICAL SAMPLES
  • TRACE-ELEMENTS
  • TISSUE
  • STRATEGIES
  • FIXATION
  • SECTION
  • METALS
  • ZINC

Cite this

Pessôa, Gustavo S. ; Capelo-Martínez, José L. ; Fdez-Riverola, Florentino ; López-Fernández, Hugo ; Glez-Peña, Daniel ; Reboiro-Jato, Miguel ; Arruda, Marco A. Z. / Laser ablation and inductively coupled plasma mass spectrometry focusing on bioimaging from elemental distribution using MatLab software : A practical guide. In: Journal of Analytical Atomic Spectrometry. 2016 ; Vol. 31, No. 4. pp. 832-840.
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title = "Laser ablation and inductively coupled plasma mass spectrometry focusing on bioimaging from elemental distribution using MatLab software: A practical guide",
abstract = "The parameters influencing laser ablation inductively coupled plasma mass spectrometry as a tool for imaging elemental distribution in tissues are critically commented on in this work, and penile cancer tissue was used as a model. General aspects of LA-ICP-MS are discussed, and among them, issues regarding sample preparation and calibration. The optimization process of the following variables is described in detail in a step-by-step tutorial manner: laser intensity, laser frequency, laser resolution, ICP radiofrequency, nebulizer and auxiliary gas flow rates, and C isotopes as internal standards. Once the best condition is found for each variable, MATLAB software is used to generate two-dimensional images. Herein it is also explained how to use MATLAB software to generate tissue images using acquisition, exporting and data treatment parameters.",
keywords = "LA-ICP-MS, SOLUTION-BASED CALIBRATION, BIOLOGICAL SAMPLES, TRACE-ELEMENTS, TISSUE, STRATEGIES, FIXATION, SECTION, METALS, ZINC",
author = "Pess{\^o}a, {Gustavo S.} and Capelo-Mart{\'i}nez, {Jos{\'e} L.} and Florentino Fdez-Riverola and Hugo L{\'o}pez-Fern{\'a}ndez and Daniel Glez-Pe{\~n}a and Miguel Reboiro-Jato and Arruda, {Marco A. Z.}",
note = "The authors thank the Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP, Sao Paulo, Brazil), the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq, Brasilia, Brazil), and Cordenadoria de Aperfeicoamento de Pessoal de Nivel Superior (CAPES, Brasilia, Brazil) for financial support. The authors are also thankful to Prof. Silvia R. Rogatto for providing the sample material. H. Lopez-Fernandez was supported by a pre-doctoral fellowship from Xunta de Galicia.",
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Laser ablation and inductively coupled plasma mass spectrometry focusing on bioimaging from elemental distribution using MatLab software : A practical guide. / Pessôa, Gustavo S.; Capelo-Martínez, José L.; Fdez-Riverola, Florentino; López-Fernández, Hugo; Glez-Peña, Daniel; Reboiro-Jato, Miguel; Arruda, Marco A. Z.

In: Journal of Analytical Atomic Spectrometry, Vol. 31, No. 4, 01.04.2016, p. 832-840.

Research output: Contribution to journalReview article

TY - JOUR

T1 - Laser ablation and inductively coupled plasma mass spectrometry focusing on bioimaging from elemental distribution using MatLab software

T2 - A practical guide

AU - Pessôa, Gustavo S.

AU - Capelo-Martínez, José L.

AU - Fdez-Riverola, Florentino

AU - López-Fernández, Hugo

AU - Glez-Peña, Daniel

AU - Reboiro-Jato, Miguel

AU - Arruda, Marco A. Z.

N1 - The authors thank the Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP, Sao Paulo, Brazil), the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq, Brasilia, Brazil), and Cordenadoria de Aperfeicoamento de Pessoal de Nivel Superior (CAPES, Brasilia, Brazil) for financial support. The authors are also thankful to Prof. Silvia R. Rogatto for providing the sample material. H. Lopez-Fernandez was supported by a pre-doctoral fellowship from Xunta de Galicia.

PY - 2016/4/1

Y1 - 2016/4/1

N2 - The parameters influencing laser ablation inductively coupled plasma mass spectrometry as a tool for imaging elemental distribution in tissues are critically commented on in this work, and penile cancer tissue was used as a model. General aspects of LA-ICP-MS are discussed, and among them, issues regarding sample preparation and calibration. The optimization process of the following variables is described in detail in a step-by-step tutorial manner: laser intensity, laser frequency, laser resolution, ICP radiofrequency, nebulizer and auxiliary gas flow rates, and C isotopes as internal standards. Once the best condition is found for each variable, MATLAB software is used to generate two-dimensional images. Herein it is also explained how to use MATLAB software to generate tissue images using acquisition, exporting and data treatment parameters.

AB - The parameters influencing laser ablation inductively coupled plasma mass spectrometry as a tool for imaging elemental distribution in tissues are critically commented on in this work, and penile cancer tissue was used as a model. General aspects of LA-ICP-MS are discussed, and among them, issues regarding sample preparation and calibration. The optimization process of the following variables is described in detail in a step-by-step tutorial manner: laser intensity, laser frequency, laser resolution, ICP radiofrequency, nebulizer and auxiliary gas flow rates, and C isotopes as internal standards. Once the best condition is found for each variable, MATLAB software is used to generate two-dimensional images. Herein it is also explained how to use MATLAB software to generate tissue images using acquisition, exporting and data treatment parameters.

KW - LA-ICP-MS

KW - SOLUTION-BASED CALIBRATION

KW - BIOLOGICAL SAMPLES

KW - TRACE-ELEMENTS

KW - TISSUE

KW - STRATEGIES

KW - FIXATION

KW - SECTION

KW - METALS

KW - ZINC

UR - http://www.scopus.com/inward/record.url?scp=84962448827&partnerID=8YFLogxK

U2 - 10.1039/c5ja00451a

DO - 10.1039/c5ja00451a

M3 - Review article

VL - 31

SP - 832

EP - 840

JO - Journal of Analytical Atomic Spectrometry

JF - Journal of Analytical Atomic Spectrometry

SN - 0267-9477

IS - 4

ER -