Abstract
The parameters influencing laser ablation inductively coupled plasma mass spectrometry as a tool for imaging elemental distribution in tissues are critically commented on in this work, and penile cancer tissue was used as a model. General aspects of LA-ICP-MS are discussed, and among them, issues regarding sample preparation and calibration. The optimization process of the following variables is described in detail in a step-by-step tutorial manner: laser intensity, laser frequency, laser resolution, ICP radiofrequency, nebulizer and auxiliary gas flow rates, and C isotopes as internal standards. Once the best condition is found for each variable, MATLAB software is used to generate two-dimensional images. Herein it is also explained how to use MATLAB software to generate tissue images using acquisition, exporting and data treatment parameters.
Original language | English |
---|---|
Pages (from-to) | 832-840 |
Number of pages | 9 |
Journal | Journal of Analytical Atomic Spectrometry |
Volume | 31 |
Issue number | 4 |
DOIs | |
Publication status | Published - 1 Apr 2016 |
Keywords
- LA-ICP-MS
- SOLUTION-BASED CALIBRATION
- BIOLOGICAL SAMPLES
- TRACE-ELEMENTS
- TISSUE
- STRATEGIES
- FIXATION
- SECTION
- METALS
- ZINC