Laser ablation and inductively coupled plasma mass spectrometry focusing on bioimaging from elemental distribution using MatLab software

A practical guide

Gustavo S. Pessôa, José L. Capelo-Martínez, Florentino Fdez-Riverola, Hugo López-Fernández, Daniel Glez-Peña, Miguel Reboiro-Jato, Marco A. Z. Arruda

Research output: Contribution to journalReview article

13 Citations (Scopus)

Abstract

The parameters influencing laser ablation inductively coupled plasma mass spectrometry as a tool for imaging elemental distribution in tissues are critically commented on in this work, and penile cancer tissue was used as a model. General aspects of LA-ICP-MS are discussed, and among them, issues regarding sample preparation and calibration. The optimization process of the following variables is described in detail in a step-by-step tutorial manner: laser intensity, laser frequency, laser resolution, ICP radiofrequency, nebulizer and auxiliary gas flow rates, and C isotopes as internal standards. Once the best condition is found for each variable, MATLAB software is used to generate two-dimensional images. Herein it is also explained how to use MATLAB software to generate tissue images using acquisition, exporting and data treatment parameters.

Original languageEnglish
Pages (from-to)832-840
Number of pages9
JournalJournal of Analytical Atomic Spectrometry
Volume31
Issue number4
DOIs
Publication statusPublished - 1 Apr 2016

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Keywords

  • LA-ICP-MS
  • SOLUTION-BASED CALIBRATION
  • BIOLOGICAL SAMPLES
  • TRACE-ELEMENTS
  • TISSUE
  • STRATEGIES
  • FIXATION
  • SECTION
  • METALS
  • ZINC

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