TY - JOUR
T1 - L1cam as an e-selectin ligand in colon cancer
AU - Deschepper, Fanny M.
AU - Zoppi, Roberta
AU - Pirro, Martina
AU - Hensbergen, Paul J.
AU - Dall’olio, Fabio
AU - Kotsias, Maximillianos
AU - Gardner, Richard A.
AU - Spencer, Daniel I. R.
AU - Videira, Paula A.
N1 - info:eu-repo/grantAgreement/EC/H2020/676421/EU#
info:eu-repo/grantAgreement/FCT/5876/147258/PT#
POCI-01-0145-FEDER-007728
ref. 140_596817822
PY - 2020/11/5
Y1 - 2020/11/5
N2 - Metastasis is the main cause of death among colorectal cancer (CRC) patients. E-selectin and its carbohydrate ligands, including sialyl Lewis X (sLeX) antigen, are key players in the binding of circulating tumor cells to the endothelium, which is one of the major events leading to organ invasion. Nevertheless, the identity of the glycoprotein scaffolds presenting these glycans in CRC remains unclear. In this study, we firstly have characterized the glycoengineered cell line SW620 transfected with the fucosyltransferase 6 (FUT6) coding for the α1,3-fucosyltransferase 6 (FUT6), which is the main enzyme responsible for the synthesis of sLeX in CRC. The SW620FUT6 cell line expressed high levels of sLeX antigen and E-selectin ligands. Moreover, it displayed increased migration ability. E-selectin ligand glycoproteins were isolated from the SW620FUT6 cell line, identified by mass spectrometry, and validated by flow cytometry and Western blot (WB). The most prominent E-selectin ligand we identified was the neural cell adhesion molecule L1 (L1CAM). Previous studies have shown association of L1CAM with metastasis in cancer, thus the novel role as E-selectin counter-receptor contributes to understand the molecular mechanism involving L1CAM in metastasis formation.
AB - Metastasis is the main cause of death among colorectal cancer (CRC) patients. E-selectin and its carbohydrate ligands, including sialyl Lewis X (sLeX) antigen, are key players in the binding of circulating tumor cells to the endothelium, which is one of the major events leading to organ invasion. Nevertheless, the identity of the glycoprotein scaffolds presenting these glycans in CRC remains unclear. In this study, we firstly have characterized the glycoengineered cell line SW620 transfected with the fucosyltransferase 6 (FUT6) coding for the α1,3-fucosyltransferase 6 (FUT6), which is the main enzyme responsible for the synthesis of sLeX in CRC. The SW620FUT6 cell line expressed high levels of sLeX antigen and E-selectin ligands. Moreover, it displayed increased migration ability. E-selectin ligand glycoproteins were isolated from the SW620FUT6 cell line, identified by mass spectrometry, and validated by flow cytometry and Western blot (WB). The most prominent E-selectin ligand we identified was the neural cell adhesion molecule L1 (L1CAM). Previous studies have shown association of L1CAM with metastasis in cancer, thus the novel role as E-selectin counter-receptor contributes to understand the molecular mechanism involving L1CAM in metastasis formation.
KW - Colorectal cancer
KW - E-selectin ligand
KW - L1CAM
KW - Sialyl Lewis X antigen
UR - http://www.scopus.com/inward/record.url?scp=85095739149&partnerID=8YFLogxK
U2 - 10.3390/ijms21218286
DO - 10.3390/ijms21218286
M3 - Article
C2 - 33167483
AN - SCOPUS:85095739149
SN - 1661-6596
VL - 21
SP - 1
EP - 23
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
IS - 21
M1 - 8286
ER -