TY - JOUR
T1 - Kinetics of cytokine expression in mice with invasive aspergillosis
T2 - Lethal infection and protection
AU - Centeno-Lima, Sónia
AU - Silveira, Henrique
AU - Casimiro, Catarina
AU - Aguiar, Pedro
AU - Rosário, Virgílio E.
PY - 2002/1/14
Y1 - 2002/1/14
N2 - Aspergillus fumigatus causes lethal invasive aspergillosis in cortisone-treated mice while immunized mice are protected. Kinetics of interferon (IFN)-γ, tumor necrosis factor-α, interleukin (IL)-12p40, IL-4 and IL-10 expression were assessed by reverse transcription-PCR in the spleen and lungs of mice. After immunization, mice were protected from otherwise lethal infection, with concomitant high expression levels of IFN-γ early in the lungs and later in the spleen. In the lethal infection model cortisone-treated infected mice presented high expression levels of IL-10 in the spleen and in the lungs. Immunocompetent mice were able to control the disease and presented earlier expression of IL-12p40 and IFN-γ in lungs and spleen. These results confirm the importance of Th1 dependent immunity against invasive aspergillosis.
AB - Aspergillus fumigatus causes lethal invasive aspergillosis in cortisone-treated mice while immunized mice are protected. Kinetics of interferon (IFN)-γ, tumor necrosis factor-α, interleukin (IL)-12p40, IL-4 and IL-10 expression were assessed by reverse transcription-PCR in the spleen and lungs of mice. After immunization, mice were protected from otherwise lethal infection, with concomitant high expression levels of IFN-γ early in the lungs and later in the spleen. In the lethal infection model cortisone-treated infected mice presented high expression levels of IL-10 in the spleen and in the lungs. Immunocompetent mice were able to control the disease and presented earlier expression of IL-12p40 and IFN-γ in lungs and spleen. These results confirm the importance of Th1 dependent immunity against invasive aspergillosis.
KW - Aspergillus fumigatus
KW - Reverse transcription-PCR
KW - Th1/Th2 cytokine
UR - http://www.scopus.com/inward/record.url?scp=0037074524&partnerID=8YFLogxK
UR - https://onlinelibrary.wiley.com/doi/epdf/10.1111/j.1574-695X.2002.tb00549.x
U2 - 10.1016/S0928-8244(01)00297-8
DO - 10.1016/S0928-8244(01)00297-8
M3 - Article
C2 - 11821239
AN - SCOPUS:0037074524
SN - 0928-8244
VL - 32
SP - 167
EP - 173
JO - Fems Immunology And Medical Microbiology
JF - Fems Immunology And Medical Microbiology
IS - 2
ER -