Jaminaea phylloscopi sp. Nov. (microstromatales), a basidiomycetous yeast isolated from migratory birds in the mediterranean basin

Nicola Francesca, Marco Alexandre Guerreiro, Cláudia Carvalho, Marco Coelho, Antonio Alfonzo, Walter Randazzo, José Paulo Sampaio, Giancarlo Moschetti

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

During a survey of yeasts vectored by migratory birds in the Mediterranean basin, isolations from the cloacae of members of the order Passeriformes collected in Ustica (Italy) were performed. Based on phylogenetic analysis of the D1/D2 domain of the 26S rRNA gene and the internal transcribed spacer ITS1-5.8S rRNA gene-ITS2 region, five yeast isolates clustered in a new lineage within the Microstromatales clade. The DNA sequences of these isolates differed from those of their closest relatives, Jaminaea angkorensis and Jaminaea lanaiensis, by 20 and 25 nt substitutions in the D1/D2 domain and 119 and 131 nt substitutions in the complete ITS region, respectively. In addition, the five isolates showed phenotypic characteristics not observed in their closest relatives, such as the ability to grow at 44 °C and at pH 2.5, which suggests a possible adaptation to the bird gastrointestinal tract. On the basis of the isolation source, phenotypic features and molecular strain typing carried out with randomly amplified polymorphic DNA (RAPD)-PCR and mini-satellite-primed (MSP)-PCR analysis, the five isolates were characterized as five distinct strains of a novel species formally described as Jaminaea phylloscopi sp. nov., with 551B6T (=PYCC 6783T=CBS 14087T) as the type strain. The Mycobank accession number is MB811984.

Original languageEnglish
Article number000801
Pages (from-to)824-829
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume66
Issue number2
DOIs
Publication statusPublished - 1 Feb 2016

Fingerprint

Microstromatales
rRNA Genes
Birds
yeast
substitution
Passeriformes
Yeasts
basins
Cloaca
yeasts
DNA
Molecular Typing
Polymerase Chain Reaction
gene
basin
ribosomal RNA
Italy
Gastrointestinal Tract
cloaca
bird

Keywords

  • SYMPODIOMYCOPSIS-LANAIENSIS
  • CRYPTOCOCCUS-NEOFORMANS
  • ANAMORPHIC FUNGUS
  • AUSTRALIA
  • GENUS
  • F.A
  • DNA

Cite this

Francesca, Nicola ; Guerreiro, Marco Alexandre ; Carvalho, Cláudia ; Coelho, Marco ; Alfonzo, Antonio ; Randazzo, Walter ; Sampaio, José Paulo ; Moschetti, Giancarlo. / Jaminaea phylloscopi sp. Nov. (microstromatales), a basidiomycetous yeast isolated from migratory birds in the mediterranean basin. In: International Journal of Systematic and Evolutionary Microbiology. 2016 ; Vol. 66, No. 2. pp. 824-829.
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abstract = "During a survey of yeasts vectored by migratory birds in the Mediterranean basin, isolations from the cloacae of members of the order Passeriformes collected in Ustica (Italy) were performed. Based on phylogenetic analysis of the D1/D2 domain of the 26S rRNA gene and the internal transcribed spacer ITS1-5.8S rRNA gene-ITS2 region, five yeast isolates clustered in a new lineage within the Microstromatales clade. The DNA sequences of these isolates differed from those of their closest relatives, Jaminaea angkorensis and Jaminaea lanaiensis, by 20 and 25 nt substitutions in the D1/D2 domain and 119 and 131 nt substitutions in the complete ITS region, respectively. In addition, the five isolates showed phenotypic characteristics not observed in their closest relatives, such as the ability to grow at 44 °C and at pH 2.5, which suggests a possible adaptation to the bird gastrointestinal tract. On the basis of the isolation source, phenotypic features and molecular strain typing carried out with randomly amplified polymorphic DNA (RAPD)-PCR and mini-satellite-primed (MSP)-PCR analysis, the five isolates were characterized as five distinct strains of a novel species formally described as Jaminaea phylloscopi sp. nov., with 551B6T (=PYCC 6783T=CBS 14087T) as the type strain. The Mycobank accession number is MB811984.",
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Jaminaea phylloscopi sp. Nov. (microstromatales), a basidiomycetous yeast isolated from migratory birds in the mediterranean basin. / Francesca, Nicola; Guerreiro, Marco Alexandre; Carvalho, Cláudia; Coelho, Marco; Alfonzo, Antonio; Randazzo, Walter; Sampaio, José Paulo; Moschetti, Giancarlo.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 66, No. 2, 000801, 01.02.2016, p. 824-829.

Research output: Contribution to journalArticle

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AU - Guerreiro, Marco Alexandre

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AU - Randazzo, Walter

AU - Sampaio, José Paulo

AU - Moschetti, Giancarlo

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