Isolation and preliminary characterization of a soluble nitrate reductase from the sulfate reducing organism Desulfovibrio desulfuricans ATCC 27774

Sergey Bursakov; Ming Y. Liu; William J. Payne; Jean LeGall; Isabel Moura; José J. G. Moura

doi:10.1016/S1075-9964(95)80444-7

Isolation and preliminary characterization of a soluble nitrate reductase from the sulfate reducing organism Desulfovibrio desulfuricans ATCC 27774

Sergey Bursakov, Ming Y. Liu, William J. Payne, Jean LeGall, Isabel Moura, José J. G. Moura

Research output: Contribution to journal › Article › peer-review

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Abstract

Desulfovibrio desulfuricans ATCC 27774 is a sulfate reducer that can adapt to nitrate respiration, inducing the enzymes required to utilize this alternative metabolic pathway. Nitrite reductase from this organism has been previously isolated and characterized, but no information was available on the enzyme involved in the reduction of nitrate. This is the first report of purification to homogeneity of a nitrate reductase from a sulfate reducing organism, thus completing the enzymatic system required to convert nitrate (through nitrite) to ammonia. D. desulfuricans nitrate reductase is a monomeric (circa 70 kDa) periplasmic enzyme with a specific activity of 5.4 K_m for nitrate was estimated to be 20 μM. EPR signals due to one Z4Fe-4S] cluster and Mo(V) were identified in dithionite reduced samples and in the presence of nitrate.

Original language	English
Pages (from-to)	55-60
Number of pages	6
Journal	Anaerobe
Volume	1
Issue number	1
DOIs	https://doi.org/10.1016/S1075-9964(95)80444-7
Publication status	Published - 1 Jan 1995

Keywords

EPR
iron-sulfur centers
molybdenum
nitrate reductase
sulphate reducing bacteria

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Isolation and preliminary characterization of a soluble nitrate reductase from the sulfate reducing organism Desulfovibrio desulfuricans ATCC 27774",

abstract = "Desulfovibrio desulfuricans ATCC 27774 is a sulfate reducer that can adapt to nitrate respiration, inducing the enzymes required to utilize this alternative metabolic pathway. Nitrite reductase from this organism has been previously isolated and characterized, but no information was available on the enzyme involved in the reduction of nitrate. This is the first report of purification to homogeneity of a nitrate reductase from a sulfate reducing organism, thus completing the enzymatic system required to convert nitrate (through nitrite) to ammonia. D. desulfuricans nitrate reductase is a monomeric (circa 70 kDa) periplasmic enzyme with a specific activity of 5.4 Km for nitrate was estimated to be 20 μM. EPR signals due to one Z4Fe-4S] cluster and Mo(V) were identified in dithionite reduced samples and in the presence of nitrate.",

keywords = "EPR, iron-sulfur centers, molybdenum, nitrate reductase, sulphate reducing bacteria",

author = "Sergey Bursakov and Liu, {Ming Y.} and Payne, {William J.} and Jean LeGall and Isabel Moura and Moura, {Jos{\'e} J. G.}",

note = "This work was supported by the Junta Nacional de Investiga{\c c}{\~a}o Cientlfica e Tecnol6gica grants STRDA/BIO/359 (IM), STRDA/ C/CEN/538/2 (JM), PBICT/QUI/616 (IM) and PBICT/P/BIO/ 1668 0-M), National Science Foundation Grant MCB 91057110NJP and JL) and National institutes of Health Grant GM 39803 0L).",

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AU - LeGall, Jean

AU - Moura, Isabel

AU - Moura, José J. G.

N1 - This work was supported by the Junta Nacional de Investigação Cientlfica e Tecnol6gica grants STRDA/BIO/359 (IM), STRDA/ C/CEN/538/2 (JM), PBICT/QUI/616 (IM) and PBICT/P/BIO/ 1668 0-M), National Science Foundation Grant MCB 91057110NJP and JL) and National institutes of Health Grant GM 39803 0L).

PY - 1995/1/1

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N2 - Desulfovibrio desulfuricans ATCC 27774 is a sulfate reducer that can adapt to nitrate respiration, inducing the enzymes required to utilize this alternative metabolic pathway. Nitrite reductase from this organism has been previously isolated and characterized, but no information was available on the enzyme involved in the reduction of nitrate. This is the first report of purification to homogeneity of a nitrate reductase from a sulfate reducing organism, thus completing the enzymatic system required to convert nitrate (through nitrite) to ammonia. D. desulfuricans nitrate reductase is a monomeric (circa 70 kDa) periplasmic enzyme with a specific activity of 5.4 Km for nitrate was estimated to be 20 μM. EPR signals due to one Z4Fe-4S] cluster and Mo(V) were identified in dithionite reduced samples and in the presence of nitrate.

AB - Desulfovibrio desulfuricans ATCC 27774 is a sulfate reducer that can adapt to nitrate respiration, inducing the enzymes required to utilize this alternative metabolic pathway. Nitrite reductase from this organism has been previously isolated and characterized, but no information was available on the enzyme involved in the reduction of nitrate. This is the first report of purification to homogeneity of a nitrate reductase from a sulfate reducing organism, thus completing the enzymatic system required to convert nitrate (through nitrite) to ammonia. D. desulfuricans nitrate reductase is a monomeric (circa 70 kDa) periplasmic enzyme with a specific activity of 5.4 Km for nitrate was estimated to be 20 μM. EPR signals due to one Z4Fe-4S] cluster and Mo(V) were identified in dithionite reduced samples and in the presence of nitrate.

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KW - molybdenum

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Isolation and preliminary characterization of a soluble nitrate reductase from the sulfate reducing organism Desulfovibrio desulfuricans ATCC 27774

Abstract

Keywords

UN SDGs

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