Isolation and characterisation of a novel sulphate-reducing bacterium of the Desulfovibrio genus

Maria J. Feio, Iwona B. Beech, Marta Carepo, João M. Lopes, C. W. S. Cheung, Ricardo Franco, J. Guezennec, J. R. Smith, Julian I. Mitchell, José J. G. Moura, Ana R. Lino

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

A novel sulphate-reducing bacterium (Ind 1) was isolated from a biofilm removed from a severely corroded carbon steel structure in a marine environment. Light microscopy observations revealed that cells were Gram-negative, rod shaped and very motile. Partial 16S rRNA gene sequencing and analysis of the fatty acid profile demonstrated a strong similarity between the new species and members from the Desulfovibrio genus. This was confirmed by the results obtained following purification and characterisation of the key proteins involved in the sulphate-reduction pathway. Several metal-containing proteins, such as two periplasmic proteins: hydrogenase and cytochrome c3, and two cytoplasmic proteins: ferredoxin and sulphite reductase, were isolated and purified. The latter proved to be of the desulfoviridin type which is typical of the Desulfovibrio genus. The study of the remaining proteins revealed a high degree of similarity with the homologous proteins isolated from Desulfovibrio gigas. However, the position of the strain within the phylogenetic tree clearly indicates that the bacterium is closely related to Desulfovibrio gabonensis, and these three strains form a separate cluster in the delta subdivision of the Proteobacteria. On the basis of the results obtained, it is suggested that Ind 1 belongs to a new species of the genus Desulfovibrio, and the name Desulfovibrio indonensis is proposed.

Original languageEnglish
Pages (from-to)117-130
Number of pages14
JournalAnaerobe
Volume4
Issue number2
DOIs
Publication statusPublished - Apr 1998

Fingerprint

Desulfovibrio
Sulfates
Bacteria
Proteins
Sulfite Reductase (Ferredoxin)
Hydrogensulfite Reductase
Desulfovibrio gigas
Deltaproteobacteria
Periplasmic Proteins
Steel
Biofilms
rRNA Genes
Names
Microscopy
Fatty Acids
Carbon
Metals
Light

Keywords

  • Cytochrome c
  • Desulfovibrio
  • Ferredoxin
  • Hydrogenase
  • Sulphate-reducing bacterium
  • Sulphite reductase (desulfoviridin)

Cite this

Feio, Maria J. ; Beech, Iwona B. ; Carepo, Marta ; Lopes, João M. ; Cheung, C. W. S. ; Franco, Ricardo ; Guezennec, J. ; Smith, J. R. ; Mitchell, Julian I. ; Moura, José J. G. ; Lino, Ana R. / Isolation and characterisation of a novel sulphate-reducing bacterium of the Desulfovibrio genus. In: Anaerobe. 1998 ; Vol. 4, No. 2. pp. 117-130.
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abstract = "A novel sulphate-reducing bacterium (Ind 1) was isolated from a biofilm removed from a severely corroded carbon steel structure in a marine environment. Light microscopy observations revealed that cells were Gram-negative, rod shaped and very motile. Partial 16S rRNA gene sequencing and analysis of the fatty acid profile demonstrated a strong similarity between the new species and members from the Desulfovibrio genus. This was confirmed by the results obtained following purification and characterisation of the key proteins involved in the sulphate-reduction pathway. Several metal-containing proteins, such as two periplasmic proteins: hydrogenase and cytochrome c3, and two cytoplasmic proteins: ferredoxin and sulphite reductase, were isolated and purified. The latter proved to be of the desulfoviridin type which is typical of the Desulfovibrio genus. The study of the remaining proteins revealed a high degree of similarity with the homologous proteins isolated from Desulfovibrio gigas. However, the position of the strain within the phylogenetic tree clearly indicates that the bacterium is closely related to Desulfovibrio gabonensis, and these three strains form a separate cluster in the delta subdivision of the Proteobacteria. On the basis of the results obtained, it is suggested that Ind 1 belongs to a new species of the genus Desulfovibrio, and the name Desulfovibrio indonensis is proposed.",
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author = "Feio, {Maria J.} and Beech, {Iwona B.} and Marta Carepo and Lopes, {Jo{\~a}o M.} and Cheung, {C. W. S.} and Ricardo Franco and J. Guezennec and Smith, {J. R.} and Mitchell, {Julian I.} and Moura, {Jos{\'e} J. G.} and Lino, {Ana R.}",
note = "This work was supported by the EUREKA (CAMICO-EU 1121), INTAS and PRAXIS XXI Programmes (BD/5682/95 awarded to M. J. Feio).",
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Feio, MJ, Beech, IB, Carepo, M, Lopes, JM, Cheung, CWS, Franco, R, Guezennec, J, Smith, JR, Mitchell, JI, Moura, JJG & Lino, AR 1998, 'Isolation and characterisation of a novel sulphate-reducing bacterium of the Desulfovibrio genus', Anaerobe, vol. 4, no. 2, pp. 117-130. https://doi.org/10.1006/anae.1997.0142

Isolation and characterisation of a novel sulphate-reducing bacterium of the Desulfovibrio genus. / Feio, Maria J.; Beech, Iwona B.; Carepo, Marta; Lopes, João M.; Cheung, C. W. S.; Franco, Ricardo; Guezennec, J.; Smith, J. R.; Mitchell, Julian I.; Moura, José J. G.; Lino, Ana R.

In: Anaerobe, Vol. 4, No. 2, 04.1998, p. 117-130.

Research output: Contribution to journalArticle

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T1 - Isolation and characterisation of a novel sulphate-reducing bacterium of the Desulfovibrio genus

AU - Feio, Maria J.

AU - Beech, Iwona B.

AU - Carepo, Marta

AU - Lopes, João M.

AU - Cheung, C. W. S.

AU - Franco, Ricardo

AU - Guezennec, J.

AU - Smith, J. R.

AU - Mitchell, Julian I.

AU - Moura, José J. G.

AU - Lino, Ana R.

N1 - This work was supported by the EUREKA (CAMICO-EU 1121), INTAS and PRAXIS XXI Programmes (BD/5682/95 awarded to M. J. Feio).

PY - 1998/4

Y1 - 1998/4

N2 - A novel sulphate-reducing bacterium (Ind 1) was isolated from a biofilm removed from a severely corroded carbon steel structure in a marine environment. Light microscopy observations revealed that cells were Gram-negative, rod shaped and very motile. Partial 16S rRNA gene sequencing and analysis of the fatty acid profile demonstrated a strong similarity between the new species and members from the Desulfovibrio genus. This was confirmed by the results obtained following purification and characterisation of the key proteins involved in the sulphate-reduction pathway. Several metal-containing proteins, such as two periplasmic proteins: hydrogenase and cytochrome c3, and two cytoplasmic proteins: ferredoxin and sulphite reductase, were isolated and purified. The latter proved to be of the desulfoviridin type which is typical of the Desulfovibrio genus. The study of the remaining proteins revealed a high degree of similarity with the homologous proteins isolated from Desulfovibrio gigas. However, the position of the strain within the phylogenetic tree clearly indicates that the bacterium is closely related to Desulfovibrio gabonensis, and these three strains form a separate cluster in the delta subdivision of the Proteobacteria. On the basis of the results obtained, it is suggested that Ind 1 belongs to a new species of the genus Desulfovibrio, and the name Desulfovibrio indonensis is proposed.

AB - A novel sulphate-reducing bacterium (Ind 1) was isolated from a biofilm removed from a severely corroded carbon steel structure in a marine environment. Light microscopy observations revealed that cells were Gram-negative, rod shaped and very motile. Partial 16S rRNA gene sequencing and analysis of the fatty acid profile demonstrated a strong similarity between the new species and members from the Desulfovibrio genus. This was confirmed by the results obtained following purification and characterisation of the key proteins involved in the sulphate-reduction pathway. Several metal-containing proteins, such as two periplasmic proteins: hydrogenase and cytochrome c3, and two cytoplasmic proteins: ferredoxin and sulphite reductase, were isolated and purified. The latter proved to be of the desulfoviridin type which is typical of the Desulfovibrio genus. The study of the remaining proteins revealed a high degree of similarity with the homologous proteins isolated from Desulfovibrio gigas. However, the position of the strain within the phylogenetic tree clearly indicates that the bacterium is closely related to Desulfovibrio gabonensis, and these three strains form a separate cluster in the delta subdivision of the Proteobacteria. On the basis of the results obtained, it is suggested that Ind 1 belongs to a new species of the genus Desulfovibrio, and the name Desulfovibrio indonensis is proposed.

KW - Cytochrome c

KW - Desulfovibrio

KW - Ferredoxin

KW - Hydrogenase

KW - Sulphate-reducing bacterium

KW - Sulphite reductase (desulfoviridin)

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