Field-effect-based devices are becoming a basic structural element in a new generation of microbiosensors. Reliable molecular characterization of DNA and/or RNA is of paramount importance for disease diagnostics and to follow up alterations in gene expression profiles. The use of such devices for point-of-need diagnostics has been hindered by the need of standard or real-time PCR amplification procedures. The present work focuses on the development of a tantalum pentoxide (Ta2O5) based sensor for the real-time label free detection of DNA amplification via loop mediated isothermal amplification (LAMP) allowing for quantitative analysis of the cMYC proto-oncogene. The strategy based on the field effect sensor was tested within a range of 1 x 10(8)-10(11) copies of target DNA, and a linear relationship between the log copy number of the initial template DNA and threshold time was observed allowing for a semi-quantitative analysis of DNA template. The concept offers many of the advantages of isothermal quantitative real-time DNA amplification in a label free approach and may pave the way to point-of-care quantitative molecular analysis focused on ease of use and low cost.
- Isothermal amplification
- Ion sensitive field effect sensors
- Tantalum pentoxide
- Real time
- Label free
Veigas, B., Branquinho, R., Vaz Pinto, J., Wojcik, P. J., Martins, R. F. D. P., Fortunato, E. M. C., & Baptista, P. M. R. V. (2014). Ion sensing (EIS) real-time quantitative monitorization of isothermal DNA amplification. Biosensors & Bioelectronics, 52(NA), 50-55. https://doi.org/10.1016/j.bios.2013.08.029