INVOLVEMENT of the UBIQUITIN MEDIATED PROTEOLYTIC PATHWAY in the RESPONSE of Lemnu minor to ARSENITE

Cláudia Santos, Ricardo Manuel Boavida Ferreira, Artur Ricardo Nascimento Teixeira

Research output: Contribution to journalArticle

Abstract

Heavy metals are potent poisons for living cells. The reason for their toxicity in vivo remains uncertain but it is well known that they contribute to the accumulation of aberrant proteins (Chen and Piper, 1995, BBA, vol. 1268, pp. 59). The synthesis of heat shock proteins (hsp), a universal response of cells to heatshock, is also activated by treatments with heavy metals (Edelman et al., 1988, Plant Physiol., vol.86, pp.1048). Stressed cells also activate several components of the proteolytic system dependent on ubiquitin, that normally functions to turnover cytoplasmic and nuclear proteins. The involvement of this pathway on the response of the heat shock is well known and recently the resistance of yeast to cadmium has been related with the ubiquitin proteolytic pathway (Jungmann et al., 1993, Nature, vol. 361, pp. 369) In our work we followed the changes in both free ubiquitin and conjugated ubiquitin during the incubation of Lemna minor L. fronds in the presence of arsenite concentrations that confer thermotolerance to the plants. The observed increased levels of ubiquitin conjugates, detected by immunoblotting using antiubiquitin antibodies, suggests an involvement of the ubiquitin-mediated proteolytic pathway during arsenite treatment. This work was supported by grant PRAXIS XXI/BD/5699/95.
Original languageEnglish
Pages (from-to)A988
Number of pages1
JournalFaseb Journal
Issue number9
Publication statusPublished - 1997

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Ubiquitin
Heavy Metals
Piper
Heat-Shock Response
Organized Financing
Poisons
Nuclear Proteins
Heat-Shock Proteins
Cadmium
Immunoblotting
Yeast
Toxicity
arsenite
Yeasts
Cells
Antibodies
Proteins

Cite this

Santos, Cláudia ; Ferreira, Ricardo Manuel Boavida ; Teixeira, Artur Ricardo Nascimento . / INVOLVEMENT of the UBIQUITIN MEDIATED PROTEOLYTIC PATHWAY in the RESPONSE of Lemnu minor to ARSENITE. In: Faseb Journal. 1997 ; No. 9. pp. A988.
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abstract = "Heavy metals are potent poisons for living cells. The reason for their toxicity in vivo remains uncertain but it is well known that they contribute to the accumulation of aberrant proteins (Chen and Piper, 1995, BBA, vol. 1268, pp. 59). The synthesis of heat shock proteins (hsp), a universal response of cells to heatshock, is also activated by treatments with heavy metals (Edelman et al., 1988, Plant Physiol., vol.86, pp.1048). Stressed cells also activate several components of the proteolytic system dependent on ubiquitin, that normally functions to turnover cytoplasmic and nuclear proteins. The involvement of this pathway on the response of the heat shock is well known and recently the resistance of yeast to cadmium has been related with the ubiquitin proteolytic pathway (Jungmann et al., 1993, Nature, vol. 361, pp. 369) In our work we followed the changes in both free ubiquitin and conjugated ubiquitin during the incubation of Lemna minor L. fronds in the presence of arsenite concentrations that confer thermotolerance to the plants. The observed increased levels of ubiquitin conjugates, detected by immunoblotting using antiubiquitin antibodies, suggests an involvement of the ubiquitin-mediated proteolytic pathway during arsenite treatment. This work was supported by grant PRAXIS XXI/BD/5699/95.",
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INVOLVEMENT of the UBIQUITIN MEDIATED PROTEOLYTIC PATHWAY in the RESPONSE of Lemnu minor to ARSENITE. / Santos, Cláudia; Ferreira, Ricardo Manuel Boavida; Teixeira, Artur Ricardo Nascimento .

In: Faseb Journal, No. 9, 1997, p. A988.

Research output: Contribution to journalArticle

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