Investigation on the anaerobic propionate degradation by Escherichia coli K12

Francesca M. Simonte, Andreas Dötsch, Lisete Galego, Cecilia Arraiano, Johannes Gescher

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Propionate is an abundant carboxylic acid in nature. Microorganisms metabolize propionate aerobically via the 2-methylcitrate pathway. This pathway depends on a series of three reactions in the citric acid cycle that leads to the conversion of succinate to oxaloacetate. Interestingly, the γ-proteobacterium Escherichia coli can use propionate as a carbon and electron source under oxic but not under anoxic conditions. RT-PCR and transcriptomic analysis revealed a posttranscriptional regulation of the prpBCDE-gene cluster encoding the necessary enzymes for propionate metabolism. The polycistronic mRNA seems to be hydrolyzed in the 3′–5′ direction under anoxic conditions. This regulatory strategy is highly constructive because the last gene of the operon encodes the first enzyme of the propionate metabolism. Further analysis revealed that RNase R is involved in the hydrolysis of the prp transcripts. Consequently, an rnr-deletion strain could metabolize propionate under anoxic conditions. To the best of our knowledge, this is the first study describing the influence of RNase R on the anaerobic metabolism of E. coli.

Original languageEnglish
Pages (from-to)55-66
Number of pages12
JournalMolecular Microbiology
Volume103
Issue number1
DOIs
Publication statusPublished - 1 Jan 2017

Fingerprint Dive into the research topics of 'Investigation on the anaerobic propionate degradation by Escherichia coli K12'. Together they form a unique fingerprint.

Cite this